Journal of
Microbiology and Antimicrobials

  • Abbreviation: J. Microbiol. Antimicrob.
  • Language: English
  • ISSN: 2141-2308
  • DOI: 10.5897/JMA
  • Start Year: 2009
  • Published Articles: 151

Full Length Research Paper

Screening of Aspergillus flavus and Aspergillus fumigatus strains for extracellular protease enzyme production

Oyeleke, S. B.1*, Egwim, E. C.2 and Auta, S. H.1
1Department of Microbiology, Federal University of Technology, Minna- Nigeria. 2Department of Biochemistry, Federal University of Technology, Minna- Nigeria
Email: [email protected]

  •  Accepted: 30 September 2010
  •  Published: 30 October 2010

Abstract

The fungal isolates identified as Aspergillus fumigatus and Aspergillus flavus isolated from a local rice husk dumpsite were screened for protease enzyme production. High yields of protease enzyme were obtained by both fungi after 144 h with concentrations of 0.96 µg/ml/min for A. flavus and 0.84 µg/ml/min for A. fumigatus. Optimum temperature for the activity of protease produced by A. flavus and A. fumigatus was at 30°C with protease activities of 0.46 and 0.43 µg/ml/min, respectively. Optimum pH for the activity of protease produced by A. flavus and A. fumigatus were pH 8 and 5, respectively. The result showed that both isolates were good producers of extracellular protease enzymes which can be useful in industries.

 

Key words: Protease, rice husk, optimization, Aspergillus fumigatus, Aspergillus flavus