Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3693

Full Length Research Paper

Anti-inflammatory effects of Portulaca oleracea L. on the LPS-induced RAW 264.7 cells

Young-Ock Kim*
  • Young-Ock Kim*
  • Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Chungbuk 369-873, Republic of Korea.
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Sang-Won Lee
  • Sang-Won Lee
  • Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Chungbuk 369-873, Republic of Korea.
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Sae Won Na
  • Sae Won Na
  • College of Veterinary Medicine, Chonbuk National University, Republic of Korea.
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Hye Ran Park
  • Hye Ran Park
  • Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk university, Chungju, 380-701, Korea.
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Eun Suk Son
  • Eun Suk Son
  • Department of Applied Biochemistry, College of Biomedical & Health Science, Konkuk university, Chungju, 380-701, Korea.
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  •  Received: 20 September 2012
  •  Accepted: 23 March 2015
  •  Published: 25 March 2015

Abstract

Portulaca oleracea L. (Portulacaceae) has been widely used as a folk medicine in many countries. The present study investigated the effects of aqueous extract of P. oleracea (PO) on pro-inflammatory mediators secreted from lipopolysaccharide (LPS) - activated macrophage cells (RAW 264.7) as an established inflammation model. The reference drug indomethacin was used for comparison purpose. PO did not show cytotoxic effects at the concentrations tested. When RAW 264.7 macrophages were treated with PO together with LPS, a significant concentration, dependent inhibition of nitrogen oxide (NO) production was detected. Western blotting revealed that PO blocked protein expression of iNOS in LPS - stimulated RAW 264.7 macrophages, significantly. The change in the contents of PGE2, IL-6 and TNF-α were monitored by enzyme linked immunosorbent assay (ELISA). Compared with indomethacin, PO has much more potency and inhibited the production of PGE2, IL-6 and TNF-α in LPS- induced RAW 264.7 cells at concentrations of 0.05, 0.1, and 0.2 mg/ml (p < 0.05). These results suggested that PO might have a potential therapeutic effect by inhibiting the inflammation process such as arthritis.

 

Key words: Portulaca oleracea, inflammation, extract, therapeutic effect.