Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Biochemical and molecular analysis of soybean seed from Turkey

Esra Maltas1*, Nazan Dageri1, Hasibe Cingilli Vural2 and Salih Yildiz1
1Department of Chemistry, Faculty of Science, Selcuk University, Konya, Turkey. 2Department of Biology, Faculty of Science, Selcuk University, Konya, Turkey.
Email: [email protected]

  •  Accepted: 10 February 2011
  •  Published: 04 May 2011

Abstract

We aimed molecular analysis of soybean by lecithin specific primer pairs LE5/LE6. Genomic DNA was extracted from soybean by CTAB method and EZ1 nucleic acid isolation system. A sensitive qualitative detection method for soybean, using the polymerase chain reaction was developed with E5/LE6 primers, produced a 195 bp product. However, the antioxidant activity of methanolic extract of soybean from Turkey by using DPPH and ABTS radical scavenging assays, showed higher antioxidant activity with 53.19±0.87% and 45.10±0.32%, respectively. Fatty acid compositions and several phenolic acids and flavonoids of soybean extract were analysed by gas and high performenace chromatography. Data showed that, the main compounds of the extract were eriodictyol, naringenin and linoleic acid.

 

Key words: Antioxidant activity, fatty acid, flavonoid, soybean, molecular marker.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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