Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3804

Full Length Research Paper

Antibacterial, antioxidant and phytochemical investigation of Thuja orientalis leaves

Nakuleshwar Dut Jasuja1*, Suresh K. Sharma2, Richa Saxena1, Jyoti Choudhary1, Ramavtar Sharma2 and Suresh C. Joshi3
1Department of Biotechnology and Allied Sciences, Jayoti Vidyapeeth Women’s University, Jharna, Jaipur -303007, Rajasthan, India. 2Department of Botany, University of Rajasthan, Jaipur, Rajasthan, India. 3Department of Zoology, University of Rajasthan, Jaipur, Rajasthan, India.  
Email: [email protected]

  •  Accepted: 30 June 2013
  •  Published: 03 July 2013


In the present study, leaves of Thuja orientalis were powdered and extracted by soxhlet extractor in two solvent systems that is, (E1) ethyl acetate: chloroform: ethanol (40: 30: 30) and (E2) methanol: distilled water (70:30). This study conferred the screening of phytochemical constituents, antioxidant activity and antibacterial activity of crude Eand E2extract and its fractions. Antioxidant activity was carried out by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The results indicate that Eextract (70% methanolic extract) had the highest antioxidant effect (85.25% inhibition) at 100 µg/ml concentration and the crude extracts (Eand Eextract) showed significant (P ≤ 0.05) inhibitory activity against both gram positive and gram negative organisms. It was active against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Agrobacterium tumefaiens. The minimum inhibitory concentrations (MICs) of Eextract ranged from 0.40 to 0.85 mg/ml and E2 extract 0.55 to 1.15 mg/ml. The highest antibacterial potentiality was exhibited by Eextract. The fractions also exhibited antimicrobial activity against all the selected microorganisms. The study revealed that T. orientalis is a promising phytomedicine for antioxidant and antibacterial activity.


Key words: Thuja orientalis, 2,2-diphenyl-1-picrylhydrazyl (DPPH), extracts, fractions, minimum inhibitory concentrations (MICs).