Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Anti-oxidant and anti-diabetic activities of Lespedeza cuneata water extract

Bhesh Raj Sharma
  • Bhesh Raj Sharma
  • Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, Mokpo National University, Jeonnam 534-729, Republic of Korea.
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Min Suk Kim
  • Min Suk Kim
  • Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, Mokpo National University, Jeonnam 534-729, Republic of Korea.
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Takako Yokozawa
  • Takako Yokozawa
  • Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan
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Dong Young Rhyu*
  • Dong Young Rhyu*
  • Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, Mokpo National University, Jeonnam 534-729, Republic of Korea.
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  •  Received: 06 June 2014
  •  Accepted: 11 July 2014
  •  Published: 17 July 2014

Abstract

Oxidative damage induced by free radicals are the attendant causes of many chronic diseases. The purpose of this study was to investigate whether Lespedeza cuneata water extract (LCW) has anti-oxidant and anti-diabetic activities in vitro. Anti-oxidant activity was measured by superoxide anion (O2-), hydroxyl (·OH) radical, nitric oxide (NO) and peroxynitrite (ONOO-) scavenging assays. Further, radical-mediated protein oxidation was measured by an allophycocyanin assay. Alpha-glucosidase, dipeptidyl peptidase (DPP)-IV and anti-glycation assays were conducted to measure its anti-diabetic activities. LCW significantly inhibited O2-, ·OH and ONOO- formation. LCW strongly protected radical-induced allophycocyanin degradation during 60 min from the initiation of the reaction. LCW significantly inhibited α-glucosidase, DPP-IV and advanced glycation end product formation. Our results suggest that LCW had both anti-oxidant and anti-diabetic activities, and could be used for the prevention and treatment of oxidative stress and diabetes.  

 

Key words: Free radicals, dipeptidyl peptidase (DPP)-IV, α-glucosidase, Lespedeza cuneata, diabetes.

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