Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Identification of superior varieties of tea (Camellia sinensis (l.) o. kuntze) in the selected UPASI germplasm using biomarkers

S. Ramkumar1, P. Sureshkumar2*, A. K. A. Mandal3, K. Rajaram2 and P. Mohankumar1
1Plant Physiology and Biotechnology Division, UPASI TRF, UPASI Tea, Research Institute, Nirar Dam BPO, Valparai 642 127, Coimbatore District, Tamilnadu, India. 2Department of Biotechnology, Anna University of Technology, Tamil nadu, Triuchirappalli- 620 024, India. 3School of Bioscience and Technology, Vellore Institute of Technology, Vellore, Tamilnadu, India.
Email: [email protected]

  •  Accepted: 08 November 2011
  •  Published: 09 February 2012

Abstract

Biomarkers are used as a vital tool in cultivar improvement programme for woody perennial tree crops such as (Camellia sinensis (L.) O. Kuntze). Commercially important fifteen accessions were selected and investigated for total polyphenol oxidase (PPO) activity (U/mg of protein), based on the PPO activity range, the accessions were further separated into three groups, namely high (P/11/10, UPASI-16, UPASI-18, UPASI-14, I/30/17), moderate (UPASI-3, UPASI-17, UPASI-13, UPASI-1, UPASI-21) and low (I/30/9, p/11/15, I/30/30,MGL-16, MGL- 8) PPO activity. To study the pattern of genetic diversity, random amplified polymorphic DNA (RAPD) analysis was performed using twenty decamer primers. The RAPD amplification results revealed that genetic similarity (GS) among the accessions tested ranged 0.64 to 0.918 with an average of 0.28%. RAPD dendogram showed three distinct clusters of high, moderate and low using un-weighed pair-group method for arithmetic averages analysis (UPGMA) method. This genetic diversity studies on tea showed effectively for the initial assessment of partitioning the intra specific level of genetic variation correlated to the total PPO enzyme activity.

 

Key words: Camellia sinensis, random amplified polymorphic DNA (RAPD), polyphenol oxidase, genetic diversity.

Abbreviation

GS, Genetic similarity; PPO, polyphenol oxidase; RAPD, random amplified polymorphic DNA; UPASI, United Planters Association of South India; UPGMA, un-weighed pair-group method for arithmetic averages analysis.