Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Phenolics of Achillea fragrantissima growing in Egypt and its cytotoxic activity

Riham Omar Bakr*
  • Riham Omar Bakr*
  • Pharmacognosy Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza, Egypt.
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Reem Khedr Arafa
  • Reem Khedr Arafa
  • Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt. 3Pharmacology Department, National Research Center, Dokki, Egypt.
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Ahmed Mohamed Al-Abd
  • Ahmed Mohamed Al-Abd
  • Pharmacology Department, National Research Center, Dokki, Egypt.
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Hisham Mohamed Elshishtawy
  • Hisham Mohamed Elshishtawy
  • Microbial Molecular Biology Department, Agricultural Genetic Engineering Research Institute, Giza, Egypt.
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  •  Received: 02 April 2014
  •  Accepted: 23 May 2014
  •  Published: 03 June 2014

Abstract

Achillea fragrantissima Sch. Bip. (Asteraceae) has been reputed in folk medicine of the Arabia region and Egypt for the treatment of many diseases. Air dried aerial parts of A. fragrantissima were extracted with 70% aqueous ethanol and fractionated with petroleum ether, dichloromethane, n-butanol and ethyl acetate fractions. Among them, dichloromethane showed the highest activity and was subjected to separation and purification by various chromatographic techniques. Five flavones and one phenolic acid were isolated and identified as acerosin (1), cirsimaritin (2), cirsiliol (3), luteolin (4), apigenin (5) and caffeic acid (6), respectively. Acerosin, the flavone of higher concentration isolated for the first time in genus Achillea, showed promising cytotoxic and radical scavenging activity. Molecular modeling of acerosin indicated its ability to bind in a co-crystallized ligand-like manner with kinase enzyme (FAK), namely with Cys502, alongside extra binding interactions with Glu430, Lys454 and Asp564. Total protein profiling of A. fragrantissima DNA was performed using polyacrylamide gel electrophoresis (PAGE) for characterization of plant where the total number of bands recovered was 4.

 

Key words: Acerosin, hepatocellular carcinoma, molecular modeling, DPPH, kinase enzyme (FAK).

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This article is published under the terms of the Creative Commons Attribution License 4.0

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