Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3750

Full Length Research Paper

Antioxidant effect of Hymenaea courbaril L (Jatobá) sap on the healing of wounds on mice

Ruth Silva Lima da Costa
  • Ruth Silva Lima da Costa
  • Post-Graduate Program in Health Sciences on the West Amazon, Federal University of Acre - UFAC, Rio Branco - Ac, Brazil.
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David Smangoszevski Martins
  • David Smangoszevski Martins
  • Post-Graduate Program in Health Sciences on the West Amazon, Federal University of Acre - UFAC, Rio Branco - Ac, Brazil.
  • Google Scholar
Laan Diego Carvalho Peixoto
  • Laan Diego Carvalho Peixoto
  • Post-Graduate Program in Health Sciences on the West Amazon, Federal University of Acre - UFAC, Rio Branco - Ac, Brazil.
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Barbara Janaina Paula da Silva
  • Barbara Janaina Paula da Silva
  • Biological Activity Laboratory, School of Pharmaceutical Sciences, Federal University of Amazonas - UFAM, Manaus - AM, Brazil.
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Larissa Barbosa Borges
  • Larissa Barbosa Borges
  • Biological Activity Laboratory, School of Pharmaceutical Sciences, Federal University of Amazonas - UFAM, Manaus - AM, Brazil.
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Emerson Silva Lima
  • Emerson Silva Lima
  • Biological Activity Laboratory, School of Pharmaceutical Sciences, Federal University of Amazonas - UFAM, Manaus - AM, Brazil.
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Hector Henrique Ferreira Koolen
  • Hector Henrique Ferreira Koolen
  • Master’s Program in Biotechnology, Amazonas State University - UEA, Manaus AM, Brazil.
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Dionatas Ulises de Oliveira Meneguetti
  • Dionatas Ulises de Oliveira Meneguetti
  • Post-Graduate Program in Health Sciences on the West Amazon, Federal University of Acre - UFAC, Rio Branco - Ac, Brazil.
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Ana Flavia Marcal Pessoa
  • Ana Flavia Marcal Pessoa
  • Department of Surgery, University of Sao Paulo Medical School (FMUSP), Sao Paulo, Brazil.
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Romeu Paulo Martins Silva
  • Romeu Paulo Martins Silva
  • Post-Graduate Program in Health Sciences on the West Amazon, Federal University of Acre - UFAC, Rio Branco - Ac, Brazil.
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  •  Received: 16 January 2021
  •  Accepted: 24 March 2021
  •  Published: 30 April 2021

Abstract

The use of natural products with curative action is a common cultural practice in the Brazilian Amazon, but little explored scientifically. The effect of Hymenaea courbaril L sap extract and its contribution to wound healing in mice was evaluated. The antioxidant potential of the extract was studied using the radical 1,1-diphenyl-2-picrilhidrazil and 2,2'-azino-bis (3-ethyl benzothiazoline-6-sulfonic) acid radical, anion superoxide (O2-) radical tests, phenol and flavonoid content, proliferation assay, cell viability and migration. In vivo tests were performed on adult Swiss mice, submitted to back injury and treated with a formulation containing 2% sap extract, extracted with ethyl acetate. The animals were treated topically for 14 days, it was observed that the sap extract has antioxidant activity, inhibiting the production of reactive oxygen species, presented potential for the proliferation of fibroblasts and promoted cell migration. Morphometric analysis of wound closure on the 3rd day suggested that animals treated with Jatobá sap had a reduced area of injury when compared with the control group on the 3rd post-injury day for the treated group. On the 14th day, there was no difference between groups. The total closure of the wounds did not occur during the 14-day experiment, but through histological evaluation, it was found that there was re-epithelialization. The results suggest that Jatobá sap extract has the potential to induce the healing of skin wounds due to its antioxidant activity.

Key words: Hymenaea courbaril L extract, cytotoxicity, antioxidant activity, wound.