Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3830

Full Length Research Paper

Qualitative and quantitative determination of polyphenol content of Scilla bifolia

Maria-Luiza Bălăşoiu1, Daniela Călina2,4*, Laurian Vlase3 and Maria-Viorica Bubulică2
1Hermes Pharmacy, Buzău, Romania. 2Faculty of Pharmacy, University of Medicine and Pharmacy, Craiova, Romania. 3Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. 42-4 Petru Rares Street, 200638 Craiova, Romania.
Email: [email protected]

  •  Accepted: 07 March 2012
  •  Published: 30 May 2012

Abstract

High-performance liquid chromatography with mass spectrometry and UV detection was applied for qualitative of eighteen polyphenols and quantitative determination of fourteen of them in Scilla bifolia bulbs and aerial parts. Caffeic acid, p-coumaric acid, ferulic acid, luteolin and apigenin were found in all hydrolysed and unhydrolysed sample extracts. Sinapic acid was detected and quantified only in hydrolyzed extract of bulbs and aerial parts indicating that it is present only as ester.

 

Key wordsScilla bifolia, polyphenol, high-performance liquid chromatography-diode-array detection-mass spectrometry (HPLC-DAD-MS).