Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3624

Full Length Research Paper

Phytochemical screening, chromatographic profile and evaluation of antimicrobial and antioxidant activities of three species of the Cyperaceae Juss. Family

Jose Jailson Lima Bezerra
  • Jose Jailson Lima Bezerra
  • Agricultural Sciences Center, Federal University of Alagoas, Maceió, Alagoas, Brazil.
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Ticiano Gomes do Nascimento
  • Ticiano Gomes do Nascimento
  • Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceió, Alagoas, Brazil.
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Regianne Umeko Kamiya
  • Regianne Umeko Kamiya
  • Institute of Biological Sciences and Health, Federal University of Alagoas, Maceió, Alagoas, Brazil.
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Ana Paula do Nascimento Prata
  • Ana Paula do Nascimento Prata
  • Agricultural Sciences Center, Federal University of Alagoas, Maceió, Alagoas, Brazil.
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Patrícia Muniz de Medeiros
  • Patrícia Muniz de Medeiros
  • Agricultural Sciences Center, Federal University of Alagoas, Maceió, Alagoas, Brazil.
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Sâmia Andrícia Souza da Silva2
  • Sâmia Andrícia Souza da Silva2
  • Institute of Pharmaceutical Sciences, Federal University of Alagoas, Maceió, Alagoas, Brazil.
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Cecília Nascimento de Mendonça
  • Cecília Nascimento de Mendonça
  • Institute of Biological Sciences and Health, Federal University of Alagoas, Maceió, Alagoas, Brazil.
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  •  Received: 29 May 2019
  •  Accepted: 05 July 2019
  •  Published: 25 July 2019

Abstract

Some species of the family Cyperaceae have been studied extensively for presenting bioactive compounds of pharmacological interest. Thus, the present research aimed to investigate the antimicrobial, antioxidant and chemical constituents of Kyllinga odorata Vahl, Oxycaryum cubensis Poepp. & Kunth and Rhynchospora nervosa Boeck. Hydroalcoholic extracts (1:1, v:v) of aerial and underground parts. These species were used for analysis of phytochemical prospection, quantification of total flavonoid and phenol content, and for evaluation of in vitro antioxidant activity against DPPH radical (2,2-diphenyl-1-picrylhydrazyl). The ethyl acetate and chloroform phase resulting from the liquid-liquid partitioning of the extracts of K. odorata, O. cubensis and R. nervosa were evaluated in in vitro antimicrobial assays and analyzed by high performance liquid chromatography (HPLC-DAD) identification of chemical substances. From the chromatograms obtained by HPLC-DAD, substances of great pharmacological importance were identified, such as: chlorogenic acid, myricitrin, catechin, apigenin, quercetin, luteolin, chrysin and rutin. The hydroalcoholic extract of the aerial parts of R. nervosa had the highest content of flavonoids (1.521 μg EQ/μg) and total phenols (5.020 EGA/μg), suggesting a direct relation with the excellent antioxidant activity of this species (IC50 = 122.11 μg/ml). It was evidenced that the chloroform phase of O. cubensis showed the best result, inhibiting the growth of Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa with the minimum inhibitory concentration of 15.6, 31.2 and 62 μg/ml, respectively.

Key words: Cyperaceae, biological activities, natural products, medicinal plants.