Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3641

Full Length Research Paper

In vitro and in vivo antimalarial activity of Nigella sativa L. extracts

Job Oyweri
  • Job Oyweri
  • Department of Pure and Applied sciences, Technical University of Mombasa, P. O. Box 90420 – 80100, Mombasa, Kenya.
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Awadh Mohammed
  • Awadh Mohammed
  • Department of Pure and Applied sciences, Technical University of Mombasa, P. O. Box 90420 – 80100, Mombasa, Kenya.
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Rahma Udu
  • Rahma Udu
  • Department of Pure and Applied sciences, Technical University of Mombasa, P. O. Box 90420 – 80100, Mombasa, Kenya.
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Jeremiah Gathirwa
  • Jeremiah Gathirwa
  • Centre for Biotechnology Research and Development, Centre for Traditional Medicine and Drug Research; Kenya Medical Research Institute, P. O. Box 54840- 0002, Nairobi, Kenya.
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Edwin Too
  • Edwin Too
  • Centre for Biotechnology Research and Development, Centre for Traditional Medicine and Drug Research; Kenya Medical Research Institute, P. O. Box 54840- 0002, Nairobi, Kenya.
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Protus Omondi
  • Protus Omondi
  • Department of Biochemistry and Biotechnology, School of Pure and Applied Sciences, Kenyatta University, P. O. Box 43884-00100 Nairobi, Kenya.
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Francis Kimani
  • Francis Kimani
  • Centre for Biotechnology Research and Development, Centre for Traditional Medicine and Drug Research; Kenya Medical Research Institute, P. O. Box 54840- 0002, Nairobi, Kenya.
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Suhaila Hashim
  • Suhaila Hashim
  • Department of Biochemistry and Biotechnology, School of Pure and Applied sciences, Pwani University, P. O. Box 195- 80108, Kilifi, Kenya.
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Laila Abubakar
  • Laila Abubakar
  • Department of Pure and Applied sciences, Technical University of Mombasa, P. O. Box 90420 – 80100, Mombasa, Kenya.
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  •  Received: 24 September 2019
  •  Accepted: 31 October 2019
  •  Published: 31 December 2019

Abstract

The Arabs, Asians and, Traditional Health Practitioners in Mombasa county found in Kenya have been using Nigella sativa L. seeds to traditionally manage malaria associated symptoms that is, headache, fever, chills, loss of appetite among others. The present study investigated in vitro antiplasmodial, in vivo antimalarial activities and safety of different extracts of N. sativa. Five extracts obtained via aqueous extraction and sequential extraction using hexane, dichloromethane, ethyl acetate and methanol were tested against in vitro cultures of Plasmodium falciparum. The most active extracts (methanolic and ethyl acetate) were assessed for cytotoxicity and toxicity. The two active extracts were evaluated in vivo against Plasmodium berghei ANKA strain at 500, 250 and 125 mg/kg/day. On in vitro assay, methanolic and ethyl acetate extracts showed good activity with IC50 of 80.48±12.29 and 69.81±5.24 µg/ml against W2 strain and 31.93±4.31 and 53.79±6.02 µg/ml against D6 strain, respectively. The extracts exhibited weak cytotoxicity on Vero cells and high parasitemia suppression of 75.52 and 75.30% at 500 mg/kg dose of methanolic and ethyl acetate extracts respectively. Notably, there was significant decrease (p<0.001) in activity with lower doses of the extracts. The results explain the traditional use of this plant in the Middle East and Mombasa County.

Key words: Nigella sativa L. seeds extracts, Plasmodium, antimalarial activity.