By using the fluorescent probe 2′,7′-di-chlorofluorescein diacetate (DCHF-DA), 2-chloro- 1,3-dibenzothiazoline-cyclohexene (DBZTC) and 2,2,6,6-tetramethyl-1-piperidinoxyl-boron dipyrromethene (TEMPO-BDP) to mark reactive oxygen species (ROS) in cardiomyocytes (H9C2(2-1), the effect of PPM60 and SPPM60 on the production of ROS with laser scanning confocal microscope (LSCM) was detected. The results showed that PPM60 could increase the concentration of ROS, which indicated that PPM60 had an effect of inducing the production of ROS in cardiomyocytes. However, SPPM60 could reduce the concentration of ROS, so SPPM60 had an effect of eliminating ROS in cardiomyocytes.
Key words: Pine pollen, polysaccharide, sulfated polysaccharide, cardiomyocytes, reactive oxygen species (ROS).
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