Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3833

Full Length Research Paper

Effects of pine pollen polysaccharide and its sulfate on the production of ROS in cardiomyocytes

Geng Yue* and Xia Yu
The Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, Jinan 250014, P. R. China.
Email: [email protected]

  •  Accepted: 26 June 2013
  •  Published: 03 July 2013


By using the fluorescent probe 2′,7′-di-chlorofluorescein diacetate (DCHF-DA),  2-chloro- 1,3-dibenzothiazoline-cyclohexene (DBZTC) and 2,2,6,6-tetramethyl-1-piperidinoxyl-boron dipyrromethene (TEMPO-BDP) to mark reactive oxygen species (ROS) in cardiomyocytes (H9C2(2-1), the effect of PPM60 and SPPM60 on the production of ROS with laser scanning confocal microscope (LSCM) was detected. The results showed that PPM60 could increase the concentration of ROS, which indicated that PPM60 had an effect of inducing the production of ROS in cardiomyocytes. However, SPPM60 could reduce the concentration of ROS, so SPPM60 had an effect of eliminating ROS in cardiomyocytes.


Key words: Pine pollen, polysaccharide, sulfated polysaccharide, cardiomyocytes, reactive oxygen species (ROS).