Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3830

Full Length Research Paper

Optimization of extraction technique of polysaccharides from pumpkin by response surface method

Jie Sun1*, Guoyou Yin1, Peng Du2 and Lanying Chen1
1Biological Engineering Department, Henan University of urban construction, Pingdingshan, Henan, China 467044. 2Food College, Northeast Agricultural University, Harbin, Heilongjiang, China 150030.
Email: [email protected]

  •  Accepted: 09 March 2011
  •  Published: 04 June 2011

Abstract

A study on the extraction technique of pumpkin polysaccharides has been carried out. The effect of each factor such as the temperature of extraction, the time of extraction and the solvent-solid ratio on the extraction of the polysaccharides of pumpkin were investigated based on a three-level three-factor Box-Behnken design. The extraction technique parameters were optimized with response surface method. The result showed that the best technological conditions were that the extraction of temperature was 79.85, the extraction of time was 85.85 min and the solvent-solid ratio was 14.86: 1 by solving the regression equation with design-expert software. Under those conditions, the predicted value of the extraction ratio of polysaccharides of pumpkin was 12.4065%.    

 

Keywords: Pumpkin polysaccharides, extraction, response surface method (RSM).