Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3830

Full Length Research Paper

Anti-bacterial activity of Acorus calamus and some of its derivates against fish pathogen Aeromonas hydrophila

R. Bhuvaneswari and Chellam Balasundaram*
Department of Animal Science, Fish Disease Diagnostic Laboratory, School of Life Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India.
Email: [email protected]

  •  Accepted: 21 June 2007
  •  Published: 31 July 2009

Abstract

Eighteen compounds isolated from the residue of Acorus calamus were subjected to micro titer assay to find out their inhibitory concentrations (IC) against Aeromonas hydrophila. The antimicrobial potency of the substances was ranked through bioautographic assay. The second compound (F2) obtained in the third fraction, on serial dilution, exhibited the maximum antimicrobial property with IC90 values of 1.6, 0.8, 0.26 and 0.13 mg/ml respectively. The chemical structure of the active compound in F2, derived from calamus oil was predicted through detailed spectroscopic analytical procedures like 1HNMR, 13C NMR, GC-MS, UV and IR spectroscopy and found related to β Asarone. 

 

Key words: Acorus calamus, compounds isolation, in vitro screening,Aeromonas hydrophila, structure determination, essential oil fraction.

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