Full Length Research Paper
Abstract
Eighteen compounds isolated from the residue of Acorus calamus were subjected to micro titer assay to find out their inhibitory concentrations (IC) against Aeromonas hydrophila. The antimicrobial potency of the substances was ranked through bioautographic assay. The second compound (F2) obtained in the third fraction, on serial dilution, exhibited the maximum antimicrobial property with IC90 values of 1.6, 0.8, 0.26 and 0.13 mg/ml respectively. The chemical structure of the active compound in F2, derived from calamus oil was predicted through detailed spectroscopic analytical procedures like 1HNMR, 13C NMR, GC-MS, UV and IR spectroscopy and found related to β Asarone.
Key words: Acorus calamus, compounds isolation, in vitro screening,Aeromonas hydrophila, structure determination, essential oil fraction.
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