Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3672

Full Length Research Paper

Effects of Tinospora crispa aqueous extract in regulating cholesterol metabolism in human hepatoma cancer cell line (Hep G2)

Zamree Md Shah
  • Zamree Md Shah
  • Innovation and Commercialization Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
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Mohd Kamal Nik Hasan
  • Mohd Kamal Nik Hasan
  • Natural Products Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
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Shahidan Mohd Arshad
  • Shahidan Mohd Arshad
  • Innovation and Commercialization Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
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Khairul Kamilah Abdul Kadir
  • Khairul Kamilah Abdul Kadir
  • Natural Products Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
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Ihsan Safwan Kamarazaman
  • Ihsan Safwan Kamarazaman
  • Natural Products Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
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Zulkhairi Amom
  • Zulkhairi Amom
  • Faculty of Health Sciences, Universiti Teknologi Mara (UiTM), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.
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Rasadah Mat Ali
  • Rasadah Mat Ali
  • Natural Products Division, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, Malaysia.
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Daryl Jesus Arapoc
  • Daryl Jesus Arapoc
  • Malaysian Nuclear Agency (MNA), Bangi, 43000 Kajang, Selangor, Malaysia.
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  •  Received: 13 April 2017
  •  Accepted: 16 October 2017
  •  Published: 17 November 2017

Abstract

In this study, the ability of Tinospora crispa aqueous extract (TCAE) to regulate cholesterol metabolism in human hepatoma cancer cell line (Hep G2) was determined. Cytotoxic study was performed by exposing hepatoma cell (Hep G2) towards TCAE with concentration ranging from 0.002 to 20 mg/ml for 24 h at 37°C and with 5% CO2 atmosphere. Result revealed that TCAE was not toxic to the cell. The ability of TCAE to reduce cholesterol in cell culture experiment was carried out by pre-treating Hep G2 with selected concentrations of TCAE (10, 5, 2.5, 1.25 and 0.625 mg/ml) in 6-well plate before the cell was exposed to low density lipoprotein (LDL). The concentration of apolipoprotein A1 (Apo A1), lecithin-cholesterol acyltransferase (LCAT), low density lipoprotein receptor (LDLR), scavenger receptor B1 (SRB1) and hepatic Lipase (HL) which involve in reverse cholesterol transport (RCT) pathway were determined from the 6-well plate medium. The direct pathway of cholesterol synthesis was performed according to the instruction provided in HMG-CoA Reductase Assay Kit manuals. The results showed that TCAE significantly increase (p<0.05) the concentration of Apo A1, LCAT, LDLR, SRB-1 and HL. The efficacy of these activities is appreciably good when compared with standard drug simvastatin. However, TCAE showed moderate effect in controlling mevalonate pathway. These findings suggested that TCAE has the potential to reduce cholesterol metabolism in Hep G2 cancer cell lines and the pathway of TCAE action possibly more on RCT.
 
Key words: Tinospora crispa, cholesterol metabolism, reverse cholesterol transport, cytotoxic, Hep G2.