Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Isolation and characterization of antimicrobial, anti-inflammatory and chemopreventive flavones from Premna odorata Blanco

Lunesa C. Pinzon1*, Mylene M. Uy1, Kung Hong Sze2, Mingfu Wang3 and Ivan Keung Chu2
1Department of Chemistry, College of Science and Mathematics, MSU-Iligan Institute of Technology, Iligan City, Philippines. 2Department of Chemistry, Faculty of Science, University of Hong Kong, Hong Kong SAR. 3School of Biological Sciences, Faculty of Science, University of Hong Kong, Hong Kong SAR.
Email: [email protected]

  •  Accepted: 05 February 2011
  •  Published: 04 July 2011


Premna odorata Blanco (Verbenaceae) is a native tree of the Philippines where its leaves are used traditionally for vaginal irrigation and tuberculosis. It is one of the seven components of a commercialized Philippine herbal preparation called “Pito-Pito”. Its medicinal uses, however, have not been scientifically validated. This tree is not commonly cultivated and thrive in the less accessible limestone forests of the Philippines. Solvent partitioning and fractionation of the ethanolic crude extract of the leaves isolated two yellow amorphous powders. The identities of these compounds were determined by LC/MS/MS and NMR spectroscopic analyses, and their spectra were compared with literature data. The isolates were flavone aglycones which were the widespread acacetin and the non-widespread diosmetin. These flavones were isolated from the P. odorata for the first time ever. They had been reported by earlier studies to exhibit medicinal properties as antimicrobial, anti-inflammatory and chemopreventive. Thus, the current study has provided a scientific evidence of the medicinal properties of the leaves of P. odorata that could become the popular basis for the plant’s sustainable use, conservation and cultivation.


Key words: Premna odorata Blanco (Verbenaceae), “pito-pito”, “alagau”, antimicrobial, anti-inflammatory, chemopreventive, flavones, diosmetin, acacetin.