Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Molecular cloning, characterization and functional analysis of a new isopentenyl diphosphate isomerase gene (IPI) from Curcuma wenyujin

Yunyun Gao1,2, Yudan Wu1, Yanxia Xu1, Zhenming Chen3, Haifeng Li1*, XiaopuYin1*, Zhaowu Zeng1 and Rong Chen1
1Research Center of Biomedicine and Health, Hangzhou Normal University, China. 2College of Life and Environmental, Hangzhou Normal University, China. 3Institute of Sustainability, Hangzhou Normal University, China.
Email: [email protected]

  •  Accepted: 24 February 2012
  •  Published: 30 April 2012

Abstract

A new isopentenyl diphosphate isomerase gene cwipi (GenBank: GU724874) was cloned from the Curcuma Wenyujin cDNA library. The full-length cDNA contained a 708 bp open reading frame (ORF) encoding a protein of 235 amino acids with calculated molecular mass of 27.36 kDa and isoelectric point of 5.14. Phylogenetic analysis demonstrated thatcwipi has a high level similarity to other IPIs belonged to angiosperm group. The protein encoded by cwipi was subcellularly localized to the cytoplasm using TargetP prediction software. Semi-quantity RT-PCR results indicated that the transcriptional level of cwipi in roots was higher than that of in stems and leaves. Cwipi could accelerate the accumulation of β-carotene when it was expressed in Escherichia coli harbouring reconstructed carotenoid synthetic pathway. The cloning, characterization and functional analysis of cwipiwill be useful for understanding the role of IPI in isoprenoid biosynthesis of C. Wenyujin.

 

Key words: Isopentenyl diphosphate isomerase, Curcuma Wenyujin, cDNA Library.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 6 No. 16
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES