Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Effect of extracts from field and in vitro plants of Petiveria alliacea L. on plasmidial DNA

Soares B. O.
  • Soares B. O.
  • Nucleo de Biotecnologia Vegetal - Universidade do Estado do Rio de Janeiro, Rua Sao Francisco Xavier 524, PHLC, Sala 602, 20550-013 Maracana, Rio de Janeiro, Brazil.
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Oliveira M. B. N
  • Oliveira M. B. N
  • Laboratorio de Radio e Fotobiologia, Universidade do Estado Rio de Janeiro, PAPC, Avenida Vinte e Oito de Setembro 87, Fundos - 4* andar, 20551-030 Vila Isabel, Rio de Janeiro, RJ. Brazil.
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Mansur E.
  • Mansur E.
  • Nucleo de Biotecnologia Vegetal - Universidade do Estado do Rio de Janeiro, Rua Sao Francisco Xavier 524, PHLC, Sala 602, 20550-013 Maracana, Rio de Janeiro, Brazil.
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Dantas F.J.S.
  • Dantas F.J.S.
  • Laboratorio de Radio e Fotobiologia, Universidade do Estado Rio de Janeiro, PAPC, Avenida Vinte e Oito de Setembro 87, Fundos - 4* andar, 20551-030 Vila Isabel, Rio de Janeiro, RJ. Brazil.
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De Mattos J. C. P.
  • De Mattos J. C. P.
  • Laboratorio de Radio e Fotobiologia, Universidade do Estado Rio de Janeiro, PAPC, Avenida Vinte e Oito de Setembro 87, Fundos - 4* andar, 20551-030 Vila Isabel, Rio de Janeiro, RJ. Brazil.
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Caldeira-de-Araujo A.
  • Caldeira-de-Araujo A.
  • Laboratorio de Radio e Fotobiologia, Universidade do Estado Rio de Janeiro, PAPC, Avenida Vinte e Oito de Setembro 87, Fundos - 4* andar, 20551-030 Vila Isabel, Rio de Janeiro, RJ. Brazil.
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Gagliardi R. F.*
  • Gagliardi R. F.*
  • Nucleo de Biotecnologia Vegetal - Universidade do Estado do Rio de Janeiro, Rua Sao Francisco Xavier 524, PHLC, Sala 602, 20550-013 Maracana, Rio de Janeiro, Brazil.
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  •  Received: 09 May 2014
  •  Accepted: 11 September 2014
  •  Published: 17 September 2014

Abstract

Petiveria alliacea L. is a native herbaceous species from the Amazon region traditionally used in folk medicine for its various pharmacological activities. This study aimed to perform a comparative assessment of genotoxic and antigenotoxic potential of field- and in vitro-grown plants. Specimens from different populations from Rio de Janeiro State were used as donor plants for in vitro culture on Murashige and Skoog (MS) medium. Aqueous extracts from field and in vitro plants were evaluated by analyzing their effect on the integrity of pUC 9.1 plasmid. DNA genotoxic potential was assessed after treatment of plasmid DNA with increasing concentrations of aqueous extracts (1.0, 5.0 to 10.0 mg/ml) from the populations studied and the in vitro plants originated from them. The electrophoretic analysis showed changes in the structural conformation of plasmid DNA, indicating the occurrence of single- and double-strand breaks caused by the extracts, demonstrating the genotoxic potential at higher concentrations. The antigenotoxic potential was assessed based on plasmid protection induced by the extracts after DNA treatment with stannous chloride. Thus, data also pointed to a antigenotoxic effect of extracts against oxidative damage. In addition, the intensity of the effect of extracts on plasmid DNA varied according to origin of plants.

 

Key words: Antigenotoxicity, bioactivity, DNA topology, genotoxicity, micropropagation

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This article is published under the terms of the Creative Commons Attribution License 4.0

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