Abstract

Archidenron lucidum is an indigenous medicinal plant in Taiwan used for treatment of inflammatory diseases and cancer. This study was aimed to investigate its anti-cancer effect against gastrointestinal and hepatic malignancy. We found that the 50% effective concentrations of its methanol extract (MEAL) against human esophageal cancer CE81T/VGH, hepatoma HA22T/VGH and murine colorectal cancer CT26 cells were less than 5.0 µg/mL in vitro whereas those of water extract were greater than 30 µg/mL. Cell cycle arrest at G2/M phase was observed in all three cell lines treated with MEAL. Development of hypodiploidy cells suggests that apoptosis might be one of the cell death pathways of CE81T/VGH cells. Intraperitoneal injection with 1.25 mg/kg MEAL significantly inhibited syngeneic CT26 tumor growth in BALB/c mice without obvious toxicity in terms of changes in body weight, leukocyte count and plasma creatinine and alanine aminotransferase (ALT) levels. Higher dose (2.5 mg/kg) MEAL did not further increase the anti-tumor effect, but resulted in elevation of plasma ALT level. Our results indicate that optimal dose of MEAL might possess the anti-tumor effects against esophageal, hepatocellular and colorectal cancers with a relative safety profile. Accordingly, we are purifying effective and less toxic compounds from MEAL.

Key words: Archidendron lucidum (Benth.), alimentary tract cancer, hepatoma.