Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3631

Full Length Research Paper

Evaluation of cytotoxic and antitumor activity of perillaldehyde 1,2-epoxide

Luciana Nalone Andrade
  • Luciana Nalone Andrade
  • Department of Medicine, Federal University of Sergipe, 330 Governador Marcelo Déda Ave, 49400-000 Lagarto, SE, Brazil.
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Patrícia Severino
  • Patrícia Severino
  • Laboratory of Nanotechnology and Nanomedicine, Tiradentes University, 300 Murilo Dantas Ave, 49032-490 Aracaju, SE, Brazil.
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Ricardo Guimaraes Amaral
  • Ricardo Guimaraes Amaral
  • Department of Physiology, Federal University of Sergipe, Marechal Rondon Ave, 49100-000 São Cristóvão, SE, Brazil.
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Grace Anne Azevedo Doria
  • Grace Anne Azevedo Doria
  • Department of Physiology, Federal University of Sergipe, Marechal Rondon Ave, 49100-000 São Cristóvão, SE, Brazil.
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Andressa da Silva
  • Andressa da Silva
  • Department of Physiology, Federal University of Sergipe, Marechal Rondon Ave, 49100-000 São Cristóvão, SE, Brazil.
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Melina Alves
  • Melina Alves
  • Department of Physiology, Federal University of Sergipe, Marechal Rondon Ave, 49100-000 São Cristóvão, SE, Brazil.
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Ricardo Luiz Cavalcante Albuquerque Junior
  • Ricardo Luiz Cavalcante Albuquerque Junior
  • Laboratory of Morphology and Experimental Pathology, Tiradentes University, 300 Murilo Dantas Ave, 49032-490 Aracaju, SE, Brazil.
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Maria Claudia Santos Luciano
  • Maria Claudia Santos Luciano
  • Department of Physiology and Pharmacology, Federal University of Ceará, Brazil.
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Claudia O Pessoa
  • Claudia O Pessoa
  • Department of Physiology and Pharmacology, Federal University of Ceará, Brazil.
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Adriana Andrade Carvalho
  • Adriana Andrade Carvalho
  • Department of Pharmacy, Federal University of Sergipe, Marechal Rondon Ave, 49100-000 São Cristóvão, SE, Brazil.
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Damiao Pergentino de Sousa
  • Damiao Pergentino de Sousa
  • Department of Pharmaceutical Sciences, Federal University of Paraíba, Campus I - Lot. Cidade Universitaria, 58033-455 João Pessoa, PB, Brazil.
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  •  Received: 06 November 2018
  •  Accepted: 04 December 2018
  •  Published: 25 December 2018

Abstract

The study aimed to evaluate cytotoxicity and antitumor activity of perillaldehyde 1,2-epoxide (PE), a p-menthane monoterpene derivative against four human tumor cell lines ovarian cancer (OVCAR-8), colon carcinoma (HCT-116), glioblastoma (SF-295) and leukemia (HL-60) using the colorimetric MTT assay. PE showed a high degree of inhibition of cell proliferation (GI = 95.66 to 99.71%) and IC50 16.14 μM (± 1.86), 23.61 μM (± 1.13), 21.99 μM (± 2.64) and, 9.70 μM (± 1.01) against tumor cells, respectively. Then, in vivo antitumor activity of the PE was assessed in sarcoma 180-bearing mice. Tumor growth inhibition rates were 33.4, 56.4 and 66.6% at doses of 100 and 200 mg/kg/day for the PE and 25 mg/kg/day for 5-FU intraperitoneal treatments, respectively. Toxicological effects related to the spleen, kidneys, liver, and hematological were investigated in mice submitted to treatment. Furthermore, histopathological analyses of these organs were absent of any morphological changes in the animals treated with PE. The viability of HL-60 cells was affected by perillaldehyde 1, 2-epoxide after an exposure period of 72 h when analyzed by trypan blue exclusion. PE reduced the number of viable cells associated with an increase in non-viable cells, which contributes to the increased number of dead cells in the morphological analysis. The incorporation of ethidium bromide/acridine orange, the treated cells suggests cytotoxicity via apoptosis and necrosis. So on the results, we conclude that PE presents cytotoxic and antitumoral activity through apoptotic and necrotic processes.

Key words: Essential oils, p-menthanes, natural products, cytotoxicity, antitumor activity, sarcoma 180.