Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3693

Full Length Research Paper

Sensitization with babassu mesocarp induces activation of murine splenocytes against tumor cells

Leticia Prince P. Pontes
  • Leticia Prince P. Pontes
  • Health Sciences Graduate Program, Biological and Health Sciences Center (UFMA), Brazil.
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Josemar M. F. Godinho Jr.
  • Josemar M. F. Godinho Jr.
  • Health Sciences Graduate Program, Biological and Health Sciences Center (UFMA), Brazil.
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Elza Moraes
  • Elza Moraes
  • Health Sciences Graduate Program, Biological and Health Sciences Center (UFMA), Brazil.
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Diego S. Arruda
  • Diego S. Arruda
  • Health Sciences Graduate Program, Biological and Health Sciences Center (UFMA), Brazil.
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Caroline S. C. Almeida
  • Caroline S. C. Almeida
  • Medical Faculty, Biological and Health Sciences Center, Federal University of Maranhão (UFMA), Brazil.
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Mayara C. Pinto
  • Mayara C. Pinto
  • Medical Faculty, Pinheiro Campi-Federal University of Maranhão (UFMA), Brazil.
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Graciomar C. Costa
  • Graciomar C. Costa
  • Department of Pathology, Biological and Health Sciences Center, Federal University of Maranhão (UFMA), Brazil.
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Rosane N. M. Guerra
  • Rosane N. M. Guerra
  • Department of Pathology, Biological and Health Sciences Center, Federal University of Maranhão (UFMA), Brazil.
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Luce M. B. Torres
  • Luce M. B. Torres
  • Botanic Institute, Center for Research in Ecology and Physiology - São Paulo, SP, Brazil.
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Vanessa F. Oliveira
  • Vanessa F. Oliveira
  • Health Sciences Center, Mogi das Cruzes University - São Paulo, SP, Brazil.
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Flávia R. F. Nascimento
  • Flávia R. F. Nascimento
  • Department of Pathology, Biological and Health Sciences Center, Federal University of Maranhão (UFMA), Brazil.
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Ana P. S. Azevedo-Santos
  • Ana P. S. Azevedo-Santos
  • Department of Physiology Sciences, Biological and Health Sciences Center, Federal University of Maranhão (UFMA), Brazil.
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  •  Received: 08 July 2016
  •  Accepted: 29 August 2016
  •  Published: 10 September 2016

Abstract

Attallea speciosa Mart. (babassu) fruit contains a mesocarp that is rich in carbohydrates with immunomodulatory effects. The induction of the tolerogenic response is a tumor escape mechanism, and immunomodulator adjuvants have been studied to reestablish host immunogenicity. This study evaluates the adjuvant potential of babassu mesocarp carbohydrates in a tumor model. The babassu mesocarp extraction (BME) yield was 75.54%, and the total sugar concentration was 29.79 mg ml-1 containing monosaccharides, reducing sugars, polysaccharides and 0.506 mg ml-1 total protein. Chromatography analysis identified glucose, sucrose and fructose. Sensitization increased the spleen weight in the tumor group compared with the control, and a comparatively lower frequency of T helper and higher frequency of B-lymphocytes was also observed. The tumor+BME group had more cytotoxic T lymphocytes compared with the control. After co-culture with cancer cells, the tumor splenocytes showed lower proliferation, lower frequency of T helper cells and higher concentrations of interleukin (IL)-2, IL-6 and IL-10. However, the tumor+BME splenocytes presented results similar to the control, suggesting a reduction in the regulatory response of the tumor group. These results demonstrated that BME sensitization with cancer cells modulated an immune response in Balb/c animals, indicating an immunogenic effect.

Key words: Arecaceae, Attallea speciosa Mart., adjuvant, carbohydrate, antitumor.