Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Effect of ginseng polysaccharide on TNF-α and IFN-γ produced by enteric mucosal lymphocytes in collagen induced arthritic rats

Hongyan Zhao1, Wandong Zhang2, Cheng Xiao3, Cheng Lu4, Shaohua Xu4, Xiaojun He4, Xiaobin Li4, Shilin Chen5, Dajian Yang5, Albert Sun-chi Chan5 and Aiping Lu4,6*
1Institute of Basic Theory, China Academy of Traditional Chinese Medicine, Beijing 100700, China. 2The First Affiliated Hospital, Anhui College of TCM, Anhui Hefei 230031, China. 3Institute of Clinical Medicine, Sino-Japan Friendship Hospital, Beijing 100009, China. 4Institute of Basic Research in Clinical Medicine, China Academy of Traditional Chinese Medicine, Beijing 100700, China. 5Institute of Modern Chinese Medicine, the Hong Kong Polytechnic University, Shenzhen 518057, China. 6E-Institute of Shanghai Municipal Education Commission, Shanghai TCM University, Shanghai, P. R., China.
Email: [email protected]

  •  Accepted: 25 May 2010
  •  Published: 04 May 2011

Abstract

To investigate the effects of ginseng polysaccharide on T-lymphocytes of enteric mucosal lymphocytes in rats with collagen induced arthritis (CIA). The peyer’s patche lymphocyte (PPL), intraepithelial lymphocyte (IEL) and lamina propria lymphocyte (LPL) of SD rats were isolated. The PPL, IEL and LPL were checked with flow cytometry and these lymphocytes were co-cultured with ginseng polysaccharide separately at different dosages. ELISA measured the expression levels of TNF-α and IFN-γ in supernatants. The results showed thatginseng polysaccharide could increased the protein expression of TNF-α and IFN-γ in the supernatant of PPL on normal rats, and it could lower the protein expression of TNF-α while increase the IFN-γ protein level in the supernatant of PPL on CIA rats. Ginseng polysaccharide could lower the protein levels both TNF-α and IFN-γ in the supernatant of LPL on normal rats. And it could lower the protein levels of TNF-α and IFN-γ in the supernatant of both IEL and LPL on CIA rats. Ginseng polysaccharide could decrease the expression of TNF-α and IFN-γ, regulate the function of Lymphocytes in enteric mucosal immune system in CIA rats, which indicate that Ginseng polysaccharide could be used in the treatment of autoimmune disease.

 

Key words: Ginseng polysaccharide, collagen induced arthritis, lymphocyte, enteric mucosal immunity, TNF-α, IFN-γ.

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