Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3753

Full Length Research Paper

Assessment of genetic diversity of Gloriosa superba L. accessions detected by random amplified polymorphic DNA analysis

Chitra Rajagopal* and Rajamani Kandhasamy
Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore - 641003, Tamil Nadu, India.
Email: [email protected]

  •  Accepted: 12 July 2013
  •  Published: 25 July 2013

Abstract

Genetic diversity of eighteen glory lily (Gloriosa superba L.) accessions of diverse geographical origin was studied using Random Amplified Polymorphic DNA (RAPD) markers. Fifty eight out of seventy primers screened showed polymorphism across the present set of accessions. A total of 413 amplicons were scored using these 58 primers. Eighty eight per cent of the amplified product showed polymorphism, indicating a fair amount of variation at the DNA level among these accessions. Cluster analysis delineated the accessions into two groups.

 

Key words: Genetic diversity, Gloriosa superba, random amplification of polymorphic DNA (RAPD), unweighted pair group method using arithmetic averages (UPGMA).