Abstract

The present study was engineered to find out the anti-arthritic and thrombolytic activity of methanolic extract of Protium serratum by using in vitro method. Methanolic extract of P. serratum was assessed with the inhibition of protein denaturation model which was used to evaluate anti-arthritic potential and this extract assessed with human blood to evaluate thrombolytic effect. The extract showed remarkable anti-arthritic activity which was evaluated by the percentages of inhibition of protein denaturation in different concentration of plant extract compared to diclofenac sodium, a reference drug. The maximum percentage inhibition of protein denaturation was observed as 83.94% at 1000 μg/ml. It has significant thrombolytic effect compared to streptokinase. During assay for thrombolytic activity, the methanolic extract of P. serratum revealed 59.653 ± 8.626% lysis of clot, while standard streptokinase (SK) and water used as positive and negative controls, demonstrated 72.835 ± 5.702 and 2.725 ± 0.983% lysis of clot, respectively. These findings demonstrate that the leaves extract of P. serratum have excellent anti-arthritic and thrombolytic effect.

Key words: Protium serratum, anti-arthric, thrombolytic, % lysis of clot, protein denaturation.