Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Antibacterial and antihemolytic activities of Enteromorpha intestinalis in Caspian Sea Coast, Iran

Saeid Soltani1*, Mohammad Ali Ebrahimzadeh2, Rahil Khoshrooei3 and Zahra Rahmani4
1Department of Biology, Qaemshahr branch, Islamic Azad University, Qaemshahr, Iran. 2Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran. 3Department of Biology, Islamic Azad University, Science and Research branch, Tehran, Iran. 4Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Email: [email protected]

  •  Published: 23 January 2012

Abstract

Enteromorpha is one of the filamentous green-algal genus and has a widespread distribution in Caspian Sea Coast. This study aimed at assaying the antimicrobial activities ofEnteromorpha intestinalis in South of Caspian Sea. Antimicrobial activities of hydroalcoholic extracts of five different gram negative and positive bacteria including Staphylococcus aureus,Bacillus subtilisPseudomonas aeruginosaSalmonella typhimurium and Proteus mirabiliswere investigated. The extract was primarily screened for their possible antimicrobial effects using disc diffusion methods. The potential antibacterial activities at different concentrations of the extract were elucidated. The extract displayed variable degrees of antimicrobial activities on different bacteria. Among gram positive bacteria, the B. subtilis (with wider zones of inhibition) was found to be more sensitive than S. aureus. Among gram negative P. aeruginosa was found to be more resistant than P. mirabilis and S. typhimurium. The extract did not show any harmful effects on erythrocytes and, in fact, exhibited potent antihaemolytic activity with IC50 of 323±11.7 μg mlcompared with 235±9 μg ml-1 for vitamin C which served as positive control. Extract show antihemolytic activity against hydrogen peroxide (H2O2) induced hemolysis. Our findings suggest the possibility of using the E. intestinalis as a novel source of natural antimicrobial and antihemolytic agent for pharmaceutical industries.

 

Key words: Enteromorpha intestinalis, antibacterial activity, antihemolytic activity.

Abbreviation

DMSO, Dimethyl solphoxamide; PBS, phosphate buffered saline.