Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3674

Full Length Research Paper

Antibacterial, cytotoxic, and schistosomicidal activities of the methanolic extract from Cassia grandis L.f. (Fabaceae) stem bark and its fractions

Luciméri Paulino Machado Magalhães
  • Luciméri Paulino Machado Magalhães
  • Departamento de Antibióticos, Universidade Federal de Pernambuco, Recife, Brazil.
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Bárbara Azevedo Ramos
  • Bárbara Azevedo Ramos
  • Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Brazil.
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Márcia Vanusa da Silva
  • Márcia Vanusa da Silva
  • Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Brazil.
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Maria Tereza dos Santos Correia
  • Maria Tereza dos Santos Correia
  • Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Brazil.
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Kêsia Xisto Fonseca Ribeiro de Sena
  • Kêsia Xisto Fonseca Ribeiro de Sena
  • Departamento de Antibióticos, Universidade Federal de Pernambuco, Recife, Brazil.
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Túlio Diego da Silva
  • Túlio Diego da Silva
  • Central Analítica, Centro de Tecnologias Estratégicas do Nordeste, Recife, Brazil.
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André de Lima Aires
  • André de Lima Aires
  • Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, Brazil.
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Mônica Camelo Pessôa de Azevedo Albuquerque
  • Mônica Camelo Pessôa de Azevedo Albuquerque
  • Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, Brazil.
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Gardênia Carmen Gadelha Militão
  • Gardênia Carmen Gadelha Militão
  • Departamento de Fisiologia e Farmacologia, Universidade Federal de Pernambuco, Recife, Brazil.
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Teresinha Gonçalves da Silva
  • Teresinha Gonçalves da Silva
  • Departamento de Antibióticos, Universidade Federal de Pernambuco, Recife, Brazil.
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Renata Mendonça Araújo
  • Renata Mendonça Araújo
  • Instituto de Química, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
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Julianna Ferreira Cavalcanti de Albuquerque
  • Julianna Ferreira Cavalcanti de Albuquerque
  • Departamento de Antibióticos, Universidade Federal de Pernambuco, Recife, Brazil.
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Rafael Matos Ximenes
  • Rafael Matos Ximenes
  • Departamento de Antibióticos, Universidade Federal de Pernambuco, Recife, Brazil.
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  •  Received: 24 January 2020
  •  Accepted: 24 February 2020
  •  Published: 30 June 2020

Abstract

Cassia grandis L. (Fabaceae), a native tree from Amazon Forest, has been used in folk medicine against worms and intestinal parasites, and to treat stomach and respiratory problems, blood diseases, among others uses. This study aimed to evaluate the antioxidant, cytotoxic, antimicrobial, and schistosomicidal activities of the methanolic extract from C. grandis stem bark (CgME) and to elucidate the chemical profile of its active fractions. The antioxidant activity of C. grandis stem bark methanolic extract (CgME) and its fractions were determined by DPPH radical scavenging assay and by the total phenolics and flavonoid contents. The antimicrobial activity was performed by microdilution. The cytotoxicity against MCF-7 (breast), NCI-H292 (lung), and HL-60 (leukemia) cancer cell lines was evaluated by the MTT method. The schistosomicidal activity was investigated in vitro against adult Schistosoma mansoni couple worms. The phytochemical profile of the active fractions was determined by GC-MS and UPLC-MS. The hexane fraction from the CgME was cytotoxic to NCI-H292 and HL-60 cancer cell lines and both major compounds clionasterol and lupeol acetate, determined by GC-MS, are well known for their cytotoxicity against cancer cells. The ethyl acetate fraction (CgEF) exhibited both antibacterial, against multidrug-resistant S. aureus, and schistosomicidal activities, which could be attributed to the presence of flavonoids, such as catechin derivatives, quercetin, and luteolin in the CgEF. These results agree with the popular uses of C. grandis and should stimulate future research on this species.

Key words: Antimicrobial activity, antioxidant, Staphylococcus aureus, clinical isolates, Schistosoma mansoni, cytotoxicity.

 

Abbreviation

CgME, Cassia grandis methanolic extract; CgHF, Cassia grandis hexane fraction; CgEF, Cassia grandis ethyl acetate fraction; CgMF, Cassia grandis methanolic fraction; CFU, colony-forming unit; DPPH, 2,2-Diphenyl-1-picrylhydrazyl; GAE, gallic acid equivalent; GC-MS, Gas chromatography-mass spectrometry; HL-60, human promyelocytic leukemia cell line; MCF-7, human breast adenocarcinoma cell line; MBC, minimum bactericidal concentration; MIC, minimum inhibitory concentration; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NCI-H292, human lung mucoepidermoid carcinoma cell line; PZQ, praziquantel; QE, quercetin equivalent; TLC, Thin-layer chromatography; UPLC-MS, Ultra-performance liquid chromatography-mass spectrometry.