Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3707

Full Length Research Paper

Assessment of thrombolytic, membrane stabilizing potential and total phenolic content of Typha elephantina Roxb.

Niloy Sen
  • Niloy Sen
  • Department of Pharmacy, Noakhali Science and Technology University, Bangladesh.
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Latifa Bulbul
  • Latifa Bulbul
  • Department of Pharmacy, Noakhali Science and Technology University, Bangladesh.
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Fahad Hussain
  • Fahad Hussain
  • Department of Pharmacy, Noakhali Science and Technology University, Bangladesh.
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Mohammad Tohidul Amin
  • Mohammad Tohidul Amin
  • Department of Pharmacy, Noakhali Science and Technology University, Bangladesh.
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  •  Received: 21 May 2016
  •  Accepted: 23 September 2016
  •  Published: 10 October 2016

Abstract

In this study, the crude methanolic extracts of whole palnt of Typha elephantina Roxb. were directed for screening of thrombolytic activity, membrane stabilizing activity, and total phenolic content. Human erythrocytes were taken for the analysis of both thrombolytic and membrane stabilizing activities and here streptokinase (SK) and acetyl salicylic acid (ASA) were used as standard for both tests, respectively. The methanolic extract of whole plant demonstrated high level of thrombolytic activity at the concentration of 4 mg/ml (33.33% of clot lysis) among various concentrations such as 2, 4, 6, 8, 7 and 10 mg/ml. On the other hand, the methanolic extract at different concentrations (1, 3,  5, 7 and 9 mg/ml), dose dependently inhibited the % of haemolysis of red blood cell (RBC) in case of heat induced condition and in case of hyponotic solution induced haemolysis, the methanolic extract just protected the RBC membrane. The total phenolic content (TPC) determination was investigated as a part of antioxidant assay. For the TPC determination, Folin-Ciocalteu method was used and the total phenolic content (126.33±4.33) of methanolic extract of T. elephantina was expressed by milligram of gallic acid equivalent to per gram of extract.

Key words: Typha elephantina Roxb., thrombolytic activity, membrane stabilizing activity, total phenolic content.