Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Antioxidant and antihepatotoxic activities of ethanolic crude extract of Melia azedarach and Piper longum

P. Samudram1*, R. Vasuki2, H. Rajeshwari3, A. Geetha4 and P. Sathiya Moorthi3
1Department of Biochemistry, SRM Medical College and Research Centre, Chennai, India. 2Department of Biochemistry, Vels college of Pharmacy, Chennai, India. 3Department of Industrial Biotechnology, Dr. MGR Educational and Research Institute, Chennai, India. 4Department of Biochemistry, Bharathi Women’s College, Chennai, India.
Email: [email protected]

  •  Accepted: 12 November 2009
  •  Published: 31 December 2009


In the present investigation, the antioxidant and antihepatotoxic activities of the crude ethanolic extract of Melia azedarach and Piper longum were studied. The antioxidative activities of biherbal ethanolic extract (BHEE) were investigated employing various established in vitro systems such as total antioxidant activity in linoleic acid emulsion system and inhibitory effect on protein oxidation. Total phenolic and flavonoid content of the extract (BHEE) were also determined by a colorimetric method. The biherbal ethanolic extract also showed potent antihepatoxic activity against carbon tetrachloride – induced acute toxicity in rat liver. The BHEE at a dose level of 50 mg/kg body weight were administered to different groups of rats orally once daily for 14 days. The degree of liver protection was determined by estimating the levels of serum marker enzymes such as SGOT, SGPT, ALP, ACP and LDH. Silymarin at a dose level of 50 mg/kg was used as standard. The results revealed that BHEE has notable inhibitory activity on peroxides formation in linoleic acid emulsion system in a dose-dependent manner. Moreover, free radical-induced protein oxidation was suppressed significantly by the addition of BHEE over a range of concentration, as revealed by the marked elevation of serum marker enzyme levels in CCl4treated rats. The amelioration of liver toxicity by BHEE was evident from its significant effect on the levels of serum marker enzymes such as SGOT, SGPT, ALP, ACP and LDH. The results of this study strongly indicate that the hepatoprotective effect of the BHEE is possibly related to its marked antioxidant activity.


Key words: Hepatoprotective, marker enzymes, biherbal ethanolic extract (BHEE), carbon tetra chloride.