Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3749

Full Length Research Paper

Assessment of minimum growth conditions for in vitro conservation of Passiflora edulis Sims

Manuella Silva Oliveira Souza
  • Manuella Silva Oliveira Souza
  • Faculdade Maria Milza (FAMAM), Campus Universitário, BR 101, Km 215, Caixa Postal nº 53, CEP 44.380.000, Governador Mangabeira, BA, Brasil.
  • Google Scholar
Eldo Ciano da Silva
  • Eldo Ciano da Silva
  • Faculdade Maria Milza (FAMAM), Campus Universitário, BR 101, Km 215, Caixa Postal nº 53, CEP 44.380.000, Governador Mangabeira, BA, Brasil.
  • Google Scholar
Gabriella Silva Oliveira Souza Ciano
  • Gabriella Silva Oliveira Souza Ciano
  • Faculdade Maria Milza (FAMAM), Campus Universitário, BR 101, Km 215, Caixa Postal nº 53, CEP 44.380.000, Governador Mangabeira, BA, Brasil.
  • Google Scholar
Claudia Cecilia Blaszkowski de Jacobi
  • Claudia Cecilia Blaszkowski de Jacobi
  • Faculdade Maria Milza (FAMAM), Campus Universitário, BR 101, Km 215, Caixa Postal nº 53, CEP 44.380.000, Governador Mangabeira, BA, Brasil.
  • Google Scholar
Josemário Santana Bonsucesso
  • Josemário Santana Bonsucesso
  • Faculdade Maria Milza (FAMAM), Campus Universitário, BR 101, Km 215, Caixa Postal nº 53, CEP 44.380.000, Governador Mangabeira, BA, Brasil.
  • Google Scholar
Weliton Antonio Bastos de Almeida
  • Weliton Antonio Bastos de Almeida
  • Faculdade Maria Milza (FAMAM), Campus Universitário, BR 101, Km 215, Caixa Postal nº 53, CEP 44.380.000, Governador Mangabeira, BA, Brasil.
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Mariane de Jesus da Silva de Carvalho
  • Mariane de Jesus da Silva de Carvalho
  • Faculdade Maria Milza (FAMAM), Campus Universitário, BR 101, Km 215, Caixa Postal nº 53, CEP 44.380.000, Governador Mangabeira, BA, Brasil.
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  •  Received: 10 April 2021
  •  Accepted: 09 August 2021
  •  Published: 31 August 2021

Abstract

Passiflora edulis Sims, besides having economic and social importance, is included in the medicinal plant list that is considered of interest by the Unified Health System (Sistema Único de Saúde – SUS). Due to its high nutritional value, this species is widely used in food, cosmetics and pharmaceutical industries. It also contains carotenoids, as well as passiflorine and maracugine, which have sedative effects. The aim of this research was to assess P. edulis minimum growth conditions in different concentrations and combinations of MS culture medium and sucrose for in vitro conservation. The following variables were analyzed: Plant height; number of green and senescent leaves; and number of roots. Root height means were highest in MS media containing 30 g L-1 sucrose (8.33). With regard to plant height, the best results were obtained in MS/4 media with 15 g L-1 or 30 g L-1 sucrose, which enabled the maintenance of lower plants (5.35 and 4.91, respectively) with green leaves after 120 days in vitro culture. The MS/2 media supplemented with 15 g L-1 sucrose was the best alternative for in vitro maintenance of plants with appropriate height and number of green leaves (8.22 and 3.86, respectively). Thus, we may conclude that MS/2 with 15 g L-1 sucrose represents an efficient strategy for in vitro conservation of P. edulis plants for a culture period of 120 days and 100% survival after acclimatization.

Key words: Passion fruit, medicinal plants, in vitro culture, germplasm conservation.