Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Phenolics from Spirulina maxima: Over-production and in vitro protective effect of its phenolics on CCl4 induced hepatotoxicity

Hanaa H. Abd El-Baky1*, Farouk K. El Baz1 and Gamal S. El-Baroty2
1Plant Biochemistry Department, National Research Centre, Dokki, Cairo, Egypt. 2 Department of Biochemistry, Faculty of Agriculture, Cairo University, Egypt.
Email: [email protected]

  •  Accepted: 04 November 2008
  •  Published: 31 January 2009


This study illustrates the process of enhancing phenolics synthesis in Spirulinamaxima grown in Zarrouk’s medium supplemented with sodium nitrate (NaNO3) and/or phenylalanine (L-PA); attaining highest production were obtained in medium containing 3.77 gL-1 NaNO3 and 100 mgL-1 L-PA. HPLC profile showed the pre-sence of phenolic acids and flavonoids predominantly with gallate, chlorogenate, cinnamate, pinostrobate and p-OH-benzoates. The protective action of Spirulinaphenolic compounds (SPC) against CCl4-induced in vitro, hepato-toxicity symptoms like microsomal lipid peroxidation and hydroxyl radical formation were studied. SPC exhibited antioxidant effects on DPPH radical scavenging with IC50values ranging from 23.22 to 35.62 µg.mL-1 and inhibited CCl4-induced lipid peroxidation in hepato-microsoms model, in dose-depended manner. Their protective potential was comparable to that of standard phenolic antioxidants such as BHT, BHA and α–tocopherol (IC50 values ranged from 13.22 to 23.62 µg.mL-1).


Key wordsSpirulina maxima, antioxidant activity, carbon tetrachloride, hepato-protective effects.