Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3727

Full Length Research Paper

Phytochemical screening, mineral and proximate composition of Asteracantha longifolia leaf extracts as a quality livestock feed

Amit Kumar Dash1, Goutam Kumar Dutta1, Gyanaranjan Sahoo2*, Sumanta Kumar Mishra3, Kautuk Kumar Sardar4 and Purna Chandra Bisoi2
1Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and A.H., Indira Gandhi Krishi Viswa Vidyalaya, Anjora, Durg, Chattishgarh, Pin- 491001, India. 2Department of Veterinary Biochemistry, College of Veterinary Science and A.H., Orissa University of Agriculture and Technology, Bhubaneswar- 751003, Odisha, India. 3Department of Animal Nutrition, College of Veterinary Science and A.H., Orissa University of Agriculture and Technology, Bhubaneswar- 751003, Odisha, India. 4Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and A.H., Orissa University of Agriculture and Technology, Bhubaneswar- 751003, Odisha, India.
Email: [email protected]

  •  Accepted: 12 April 2012
  •  Published: 09 June 2012

Abstract

Fractions of hot water extract of Asteracantha longifolia Nees leaf and aerial parts were subjected to qualitative and quantitative screening of different phytochemicals and for quantitative determination of different micro and macro elements. Dried leaf powder was analyzed for proximate composition and quantitative determination of different micro and macro elements. Proximate composition of the leaf revealed 14.212±0.375% of ash, 18.413±0.258% of protein, 3.401±0.081% of ether extract and 41.205±0.573% of fiber. Qualitative phytochemical screening of fractions of hot water extract of leaves revealed the presence of alkaloids, carbohydrates, proteins, phenolic compounds and flavonoids in all the fractions in addition to presence of proteins containing aromatic amino acids in aqueous fraction. Total phenolic compounds and total antioxidants in all the three fractions were in the range of 0.094 to 0.14 mg of gallic acid equivalent mg-1 extract and 40.95 to 317.5 µM of ascorbic acid equivalent mg-1 extract, respectively. Relatively low concentration of total antioxidant was found in residual fraction. Flavonoid concentrations were found in the range of 0.17±0.03 to 3.17±0.04 mg of quercetin equivalent g-1 extract, highest being the aqueous fraction and lowest the residual fraction. The residual fraction was found to contain a higher concentration of iron (Fe), cobalt (Co), copper (Cu), zinc (Zn), calcium (Ca), magnesium (Mg) and phosphorus (P). The level of sodium (Na) and potassium (K) were higher in methanolic fraction and hot water extract, respectively. The incorporation of A. longifolia can be a potential source of antioxidant, macro and micro minerals for manufacturing nutraceutical and improving livestock feed.

 

Key words: Qualitative, quantitative, phytochemical screening, mineral, antioxidant, phenolics.