Abstract

In the present study, Rhus verniciflua extracts from various extract condition was evaluated by employing various in vitro anti-oxidant assay such as electron donation ability by 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power by Fe³⁺-Fe²⁺transformation method, and anti-lipid peroxidative effect by ferric thiocyanate. The electron donation ability of 100% ethanolic extract (94%) showed stronger activity than butylated hydroxyanisole (18%). One hundred percent ethanolic extract (0.54) showed the highest reducing power, and the other samples (0.29 to 0.44) showed stronger activity than α-tocopherol (0.13). Total phenol and flavonoid content showed the highest amount in 100% ethanolic extract (352.6 and 54.6 mg QE/g, respectively). The anti-lipid peroxidative effect of samples was greater than same concentration of α-tocopherol. Although, higher extract yields were obtained using 80% methanol, higher anti-oxidant activities was obtained by 100% ethanol extracts.

Key words: Rhus verniciflua, anti-oxidant activity, electron donation ability, reducing power, anti-lipid peroxidative effect.