Plant biotechnology enables the production of biomass under controlled conditions, providing the synthesis of raw material in a continuous and homogeneous way. The aim of the present study was to establish Hovenia dulcis callus cultures and to evaluate their antioxidant potential in comparison with wild grown and in vitro plants. The best results for compact calli were obtained with the supplementation of 1-naphthaleneacetic acid (NAA) at 2.5 mg L-1 and the use of benzylaminopurine (BAP) enhanced callus growth. The medium supplemented with 2.5 mg L-1 NAA + 0.65 mg L-1 BAP produced 115.3±28.2 mg of dry weight. The auxin NAA was responsible for the production of light-green compact callus, while picloram and 2,4-D promoted mixed (friable and compact) calli. Total polyphenols and total flavonoids were found in higher concentrations in wild grown plants, whereas the reduction capacity and DPPH radical scavenging assays recorded higher antioxidant activity in calluses extracts. The protocols established here represent a viable and effective way for producing substances with medicinal interest.
Key words: Tissue culture, total phenolics, total flavonoids, 2,2-diphenyl-1-picrylhydrazyl (DPPH), medicinal plant.
ROS, Reactive oxygen species; BAP, benzylaminopurine; KIN, 6-furfuryl-aminopurine; 2,4-D, 2,4-dichlorophenoxyacetic acid; PIC, 4-amino-3,5,6-trichloro picolinic acid; NAA, 1-naphthaleneacetic acid; DW, dry weight; DPPH, 2,2-diphenyl-1-picrylhydrazyl.
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