Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3727

Full Length Research Paper

In vitro antitumoral activities of Padina pavonia on human cervix and breast cancer cell lines

Tatjana P. Stanojković1*, Katarina Šavikin2, Gordana Zdunić2*, Zoran Kljajić3, Nadja Grozdanić1 and Jadranka Antić4    
1Institute for Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade, Serbia. 2Institute for Medicinal Plant Research, ‘‘Dr Josif Pančić’’, Tadeuša KošÄ‡uška 1, 11000 Belgrade, Serbia. 3Institute of Marine Biology, P.O.Box 69, 85 330 Kotor, Montenegro. 4Clinical Centre of Serbia, Dr Subotića 13, 11000 Belgrade, Serbia    
Email: [email protected]

  •  Accepted: 31 August 2012
  •  Published: 25 February 2013

Abstract

Marine algae represent a potent source of anticancer agents. Brown alga, Padina pavoniaL. was collected from Adriatic Sea, Montenegro. Cytotoxic and apoptosis effects were tested against human cervix (HeLa) and breast cancer (MDA-MB-453) cell lines. P. pavoniamethanol extract possesses cytotoxic activity with IC50 values 86.45 μg/ml related to HeLa cell and 74.59 μg/ml related to MDA-MB-453 cell. The extracts did not exert any significant cytotoxicity toward normal human fetal lung fibroblast cells (MRC-5). Reduction in the number of cells with distorted shapes and condensation of cytoplasm was noticed. An increase in cells containing sub-G1 amounts of DNA was observed, indicating that the tested extract induced apoptosis. Nuclear DNA laddering was noted 24 h after the HeLa cells were exposed to 2´IC50 concentration of P. pavonia methanol extract. As brown algae represent a rich source of polysaccharides and glycosides, this activity could be connected with these compounds, but also with phenolics detected in examined extract (12.2 mg gallic acid equivalents/g dry weight (GAE/g dry weight).

 

Key words: Brown alga, HeLa, MDA-MB-453, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test.