Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3832

Full Length Research Paper

Efficacy of a mouthrinse based on leaves of the neem tree (Azadirachta indica) in the treatment of patients with chronic gingivitis: A double-blind, randomized, controlled trial

Marco Antonio Botelho1,2 *, Rinaldo Araujo dos Santos2, Jose Galberto Martins3, Cintia Oliveira Carvalho1, Mabel Calina Paz2, Cláudio Azenha4, Ronaldo Sousa Ruela4, Dinalva Brito Queiroz5, Wagner Sousa Ruela4, Gloria Marinho2, Francisca Isabel Ruela5    
1Laboratory of Applied Chemistry, School of Dentistry, Faculdades Nordeste, Brazil.  2Laboratory Chemical Technologies, Federal Technological Education Center of Ceará, Brazil. 3Research Laboratory of Natural Products, Regional University of Cariri, Brazil. 4Department of Post-Graduation, School of Dentistry, Faculdades Unidas Norte de Minas, Brazil. 5Laboratory of Biotechnology, Institute of Applied Research, Brazil. 6Department of Clinical and Surgery, Federal University of Alfenas, Brazil.    
Email: [email protected]

  •  Accepted: 21 November 2008
  •  Published: 30 November 2008


The aim of this study was to compare the short-term efficacy and safety of aAzadirachta indica mouthrinse on gingival inflammation and microbial plaque, compared to 0.12% chlorhexidine. A double-masked, randomized, parallel armed study was carried out to assess the efficacy of an oral mouthrinse based on leaves of the neem tree reducing gingivitis. Study subjects were recruited from a slum in Brazil. Fifty-four subjects were enrolled and randomly assigned in two groups (26 neem group, 28 chlorhexidine control). Interventions consisted of a seven day therapy of the A. indica-based mouth rinse and chlorhexidine 0.12%, respectively. Plaque index, gingival index and gingival bleeding index were obtained at baseline, as well as after one and four weeks. Additionally, the count of cariogenic bacteria (Streptococcus mutans) in the saliva was assessed before and after treatment. All clinical index scores were reduced in both groups seven and 30 days after treatment. There was no statistically significant difference between groups in clinical and microbiological parameters. Adverse events were mild and of transient nature. This short-term study demonstrated that A. indica-based mouth rinse is highly efficacious and that it may be used as an alternative therapy in the treatment of periodontal disease.


Keywords: Azadirachta indica, gingivitis, Streptococcus mutans, neem tree, clinical trial.