Full Length Research Paper
Abstract
Bupleuri Radix is a medicinal herb that is widely used in Asia, and several Bupleurum species are used as a source of active ingredients for traditional Chinese medicines. Among these species, Bupleurum longiradiatum Turcz., is toxic and is not used as a medicinal herb. Because B. longiradiatum is morphologically similar to other Bupleurum species, it is important to distinguish between them. In other to address this problem, we developed a polymerase chain reaction (PCR) method based on internal transcribed spacer (ITS) nucleotide sequences to discriminate B. longiradiatum from Bupleurum falcatum L. andBupleurum chinense DC., which are the most frequently used medicinal Bupleurum species. The discriminatory power of the method was verified by gel and capillary electrophoresis. Three PCR primers specific to B. longiradiatum were developed for use in this assay to enable us reveal the four B. chinense haplotypes.
Key words: Bupleurum longiradiatum, medicinal herb, molecular identification, internal transcribed spacer.
Copyright © 2024 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0