Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Internal transcribed spacer-based identification of Bupleurum species used as sources of medicinal herbs

Young Hwa Kim1, Eung Soo Kim2, Byoung Seob Ko1, Md. Romij Uddin3, Seung Eun Oh4, Go Ya Choi1, Seong Wook Chae1, Hye Won Lee1, Je Hyun Lee5, Ju Young Park6 and Mi Young Lee1*
1Aging Research Center, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Korea. 2National Forensic Service, Seoul, Korea. 3Department of Crop Science, Chungnam National University, Daejeon, Korea. 4Department of Biological Sciences, Konkuk University, Seoul, Korea. 5Department of Oriental Medicine, Dongguk University, Gyeongju, Korea. 6Korea Food and Drug Administration, Cheongwon, Korea.
Email: [email protected]

  •  Accepted: 30 November 2011
  •  Published: 09 February 2012

Abstract

Bupleuri Radix is a medicinal herb that is widely used in Asia, and several Bupleurum species are used as a source of active ingredients for traditional Chinese medicines. Among these species, Bupleurum longiradiatum Turcz., is toxic and is not used as a medicinal herb. Because B. longiradiatum is morphologically similar to other Bupleurum species, it is important to distinguish between them. In other to address this problem, we developed a polymerase chain reaction (PCR) method based on internal transcribed spacer (ITS) nucleotide sequences to discriminate B. longiradiatum from Bupleurum falcatum L. andBupleurum chinense DC., which are the most frequently used medicinal Bupleurum species. The discriminatory power of the method was verified by gel and capillary electrophoresis. Three PCR primers specific to B. longiradiatum were developed for use in this assay to enable us reveal the four B. chinense haplotypes.

 

Key words: Bupleurum longiradiatum, medicinal herb, molecular identification, internal transcribed spacer.