Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3799

Full Length Research Paper

Aqueous extracts of Lentinula edodes, Pleurotus pulmonarius, and Pleurotus sajor-caju: Antifungal activity and inhibition of exoenzyme production by Candida albicans

Morgana Lüdtke Azevedo
  • Morgana Lüdtke Azevedo
  • Graduate Program in Biochemistry and Bioprospecting, Center for Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas, Pelotas, RS, Brazil.
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Carolina Lambretch Gonçalves
  • Carolina Lambretch Gonçalves
  • Graduate Program in Microbiology and Parasitology, Biology Institute, Federal University of Pelotas, Pelotas, RS, Brazil.
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Alessandro Menna Alves
  • Alessandro Menna Alves
  • Graduate Program in Dentistry, Pelotas Dental School, Federal University of Pelotas, Pelotas, RS, Brazil.
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Caroline Neugebauer Wille
  • Caroline Neugebauer Wille
  • Federal Institute of Education, Science and Technology of Rio Grande do Sul (IFSul), Campus Camaquã, RS, Brazil.
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Adriana Etges
  • Adriana Etges
  • Graduate Program in Dentistry, Pelotas Dental School, Federal University of Pelotas, Pelotas, RS, Brazil.
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Patrícia da Silva Nascente
  • Patrícia da Silva Nascente
  • Graduate Program in Microbiology and Parasitology, Biology Institute, Federal University of Pelotas, Pelotas, RS, Brazil.
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Rafael Guerra Lund
  • Rafael Guerra Lund
  • Graduate Program in Biochemistry and Bioprospecting, Center for Chemical, Pharmaceutical and Food Sciences, Federal University of Pelotas, Pelotas, RS, Brazil.
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  •  Received: 27 June 2022
  •  Accepted: 06 October 2022
  •  Published: 31 October 2022

Abstract

This study was aimed at evaluating the effects of aqueous extracts of Lentinula edodes (SHI), Pleurotus pulmonarius (PUL), and Pleurotus sajor-caju (PSC) on the growth of eight yeast species and the exo-enzyme production by Candida species after exposure to the extracts, which were prepared with the cold extraction methodology and tested against yeasts by the broth microdilution method. Proteinase and phospholipase were produced in vitro with the plate assay method using bovine serum albumin and egg yolk, respectively. The antifungal activity of the filtered mushroom extracts at different dilutions was confirmed against non-albicans Candida and Rhodotorula species. Exposing Candida isolates to extracts significantly reduced phospholipase production (p < 0.001). The antifungal activity against yeasts varied among different mushroom extracts. Moreover, SHI and PUL extracts may modulate the activity of phospholipase produced by Candida albicans. However, further studies are required to evaluate the inhibitory effect and its mechanism, as well as complementary studies with a higher number of samples tested.

Key words: Mushrooms extracts, yeasts, Candida species, antifungal activity, proteinases, phospholipases.