Abstract

A simple micropropagation protocol for the important medicinal plant species Adhatoda vasica (Vasaka) from nodal explants was developed. The young juvenile nodal explants cultured on Murashige and Skoog’s (MS) medium supplemented with CW (15% v/v) + BAP (5 mg/L) started proliferating multiple shoot buds (14±2) in 3 to 4 weeks and the shoots became 3 cm long in 6 to 8 weeks. The regenerated plants produced well developed roots on transfer to MS + IBA 1 mg/L in 10 to 12 days. The rooted plantlets were acclimatized before been transfer to soil and 80% of the transferred plants survived.

Key words: Adhatoda vasica, Vasaka, nodal explants, micropropagation.