Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Preparative separation of hyperoside of seeds extract of Saposhnikovia divaricata by high performance counter-current chromatography

Li Li, Yuge Gui, Jing Wang, Huirong Zhang, Xiaofei Zong and Chun Ming Liu*
The Central Laboratory, Changchun Normal University, Changchun, 130032, China.
Email: [email protected]

  •  Accepted: 21 December 2011
  •  Published: 09 February 2012

Abstract

Hyperoside was isolated and purified from the seeds of Saposhnikovia divaricata for the first time by high performance counter-current chromatography (HPCCC) using a solvent system of ethyl acetate-n-butanol-ethanol -water (1:1:0.1:2, v/v/v/v). By injecting ca. 500 mg of n-butanol extract of S. divaricata seeds for five times, a total of 232.7 mg of hyperoside was purified from 2.5 g of the n-butanol extract of S. divaricata seeds, at 96.3% purity as determined by high performance liquid chromatography (HPLC). The identification of the purified compound was achieved by congruent retention time, ultraviolet (UV) spectra and the data of high-performance liquid chromatography- electrospray ion source mass spectroscopy (LC-ESI-MSn) in the positive mode with that of the authentic standard and literature reports.

 

Key words: S. divaricata seeds, hyperoside, HPCCC.