Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3761

Full Length Research Paper

Influence of cytokinins, sucrose and pH on adventitious shoot regeneration of Polyscias balfouriana (Balfour aralia)

Saiqa Ilyas1*, Shagufta Naz1, Sumeera Javad2, Kiran Shehzadi2, Amna Tariq2, Neelma Munir1 and Aamir Ali3
1Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan. 2Department of Botany, Lahore College for Women University, Lahore, Pakistan. 3Department of Biological Sciences, University of Sargodha, Pakistan.
Email: [email protected]

  •  Accepted: 09 January 2012
  •  Published: 10 November 2013

Abstract

A highly reproducible and efficient in vitro shoot regeneration system was developed for Polyscias balfouriana using apical shoot tips as explants. Explants after surface sterilization were cultured on Murashige and Skoog (MS) (1962) medium supplemented with different concentrations (0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4 and 1.6 mg/l) of 6- Benzylamino purine (BAP), Thidiazuron (TDZ) and Kinetin (Kin), alone as well as in combination with indole-3-butyric acid (IBA) (0.1, 0.5, 1.0, 1.5 and 2.0 mg/l). The highest rate of shoot multiplication (15.20 ± 0.86 for the average shoot number and 6.30 ± 0.84 cm for shoot length) was achieved on MS medium supplemented with 1.2 mg/ml BAP and 0.5 mg/ml IBA. The effect of sucrose and pH of MS medium on shoot proliferation was also tested. An average of 19.2 ± 0.34 shoots per explants with 6.90 ± 0.15 cm shoot length could be obtained by optimizing these two parameters. Rooting was achieved on micro shoots using MS medium with 3.0 mg/ml IBA. The in vitro developed healthy plantlets were successfully shifted to greenhouse for acclimatization.

Key words: Cytokinins, sucrose, pH, adventitious shoot, regeneration, Polyscias balfouriana.