This study aims at assessing the in vitro activity of Cymbopogon citratus, Eucalyptus citriodora, Lippia multiflora, Melaleuca quinquenervia essential oils and the biobactericide Neco® on extended-spectrum β-lactamases producing or non-producing bacterial strains (ESBL) isolated at the Armed forces hospital of Libreville. The aromatogram and antibiogram were respectively assessed by the agar well diffusion method and agar disc method. Thus, the minimum inhibitory concentration and minimum bactericidal concentration were determined by the microdilution method in liquid medium.
The aromatogram revealed that the biobactericide Neco® induced the largest inhibition diameters (28.42 - 43.27 mm) in all strains, followed by E. citriodora (26.96 - 36.12 mm) and L. multiflora (15.32 - 41.42 mm) essential oils. In contrast, M. quinquenervia (9.83 - 26.64 mm) and C. citratus (6.82 - 14.97 mm) essential oils had the smallest inhibition diameters. Furthermore, the comparison of aromatogram and antibiogram activities generally revealed that activities are better with essential oils than with antibiotics. In addition, no significant differences were observed between ESBL producing or non-producing strains. At the end this study, the antibacterial activity of C. citratus, E. citriodora, L. multiflora, M. quinquenervia essential oils and the biobactericide Neco® were highlighted. However, the efficiency of these activities is dependent on the intrinsic composition of the plant.
Key words: Essential oils, extended spectrum β-lactamase (ESBL), multi-resistance, biobactericide, antibacterial activity.
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