Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3693

Full Length Research Paper

In vitro antibacterial activity of Cymbopogon citratus, Eucalyptus citriodora, Lippia multiflora, Melaleuca quinquenervia essential oils and Neco® on extended-spectrum ß-lactamases producing or non-producing bacterial strains

Jean-Fabrice Yala*
  • Jean-Fabrice Yala*
  • Laboratory of Molecular and Cellular Biology, Microbiology-Immunology team; Agrobiology Research Unit, University of Sciences and Techniques of Masuku (USTM), BP 067 Franceville, Gabon.
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Rolande Mabika Mabika
  • Rolande Mabika Mabika
  • Laboratory of Molecular and Cellular Biology, Microbiology-Immunology team; Agrobiology Research Unit, University of Sciences and Techniques of Masuku (USTM), BP 067 Franceville, Gabon.
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Brahima Camara
  • Brahima Camara
  • Laboratory of Plant Physiology, Faculty of Biosciences, University Félix HOUPHOUET-BOIGNY Cocody, 22 BP 582 Abidjan 22, Côte d’Ivoire.
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Alain Souza
  • Alain Souza
  • Laboratory of Molecular and Cellular Biology, Microbiology-Immunology team; Agrobiology Research Unit, University of Sciences and Techniques of Masuku (USTM), BP 067 Franceville, Gabon.
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Alexis Nicaise Lepengue
  • Alexis Nicaise Lepengue
  • Laboratory of Plant Physiology, Plant Pathology and Plant Breeding, Agrobiology Research Unit, University of Sciences and Techniques of Masuku (USTM), BP 067 Franceville, Gabon.
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Tuo Seydou
  • Tuo Seydou
  • Laboratory of Plant Physiology, Faculty of Biosciences, University Félix HOUPHOUET-BOIGNY Cocody, 22 BP 582 Abidjan 22, Côte d’Ivoire.
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Daouda Kone
  • Daouda Kone
  • Laboratory of Plant Physiology, Faculty of Biosciences, University Félix HOUPHOUET-BOIGNY Cocody, 22 BP 582 Abidjan 22, Côte d’Ivoire.
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Bertrand M’batchi
  • Bertrand M’batchi
  • Laboratory of Plant Physiology, Plant Pathology and Plant Breeding, Agrobiology Research Unit, University of Sciences and Techniques of Masuku (USTM), BP 067 Franceville, Gabon.
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  •  Received: 12 October 2016
  •  Accepted: 11 November 2016
  •  Published: 17 November 2016

Abstract

This study aims at assessing the in vitro activity of Cymbopogon citratus, Eucalyptus citriodora, Lippia multiflora, Melaleuca quinquenervia essential oils and the biobactericide Neco® on extended-spectrum β-lactamases producing or non-producing bacterial strains (ESBL) isolated at the Armed forces hospital of Libreville. The aromatogram and antibiogram were respectively assessed by the agar well diffusion method and agar disc method. Thus, the minimum inhibitory concentration and minimum bactericidal concentration were determined by the microdilution method in liquid medium.

The aromatogram revealed that the biobactericide Neco® induced the largest inhibition diameters (28.42 - 43.27 mm) in all strains, followed by E. citriodora (26.96 - 36.12 mm) and L. multiflora (15.32 - 41.42 mm) essential oils. In contrast, M. quinquenervia (9.83 - 26.64 mm) and C. citratus (6.82 - 14.97 mm) essential oils had the smallest inhibition diameters. Furthermore, the comparison of aromatogram and antibiogram activities generally revealed that activities are better with essential oils than with antibiotics. In addition, no significant differences were observed between ESBL producing or non-producing strains. At the end this study, the antibacterial activity of C. citratus, E. citriodora, L. multiflora, M. quinquenervia essential oils and the biobactericide Neco® were highlighted. However, the efficiency of these activities is dependent on the intrinsic composition of the plant.

Key words: Essential oils, extended spectrum β-lactamase (ESBL), multi-resistance, biobactericide, antibacterial activity.