Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3793

Full Length Research Paper

Antioxidant and cytotoxic activity of the acetone extracts of root of Euphorbia hylonoma and its ellagic acid derivatives

Zengjun Guo*, Ying Xu, Ling Han, Xiaoqian Bo, Chen Huang and Lei Ni
Faculty of Pharmacy, Medical School of Xi’an Jiaotong University, Xi’an 710061, China.
Email: [email protected]

  •  Accepted: 15 July 2011
  •  Published: 16 September 2011

Abstract

The acetone extracts and fractions prepared from the roots of Euphorbia hylonoma were investigated for 1, 1-diphenyl-2-pycrylhydrazyl (DPPH) free radical, superoxide anion and hydroxyl radical scavenging activity and cytotoxic activity against the human hepatoma cell line SMMC-7721, human cervix epitheloid carcinoma cell line HeLa and the human gastric cancer cell line SGC-7901. The acetone extract and fraction of EtOAc showed high antioxidant activities. The acetone extract and fraction of water showed a stronger cytotoxic effect on all tested cells, with IC50 values being less than 20 μg/mL. The acetone extract and fraction of water induced cell cycle arrest in G1 phase in SMMC-7721 cells. The major compounds isolated from the acetone extract were ellagic acid derivatives and showed weak cytotoxic activities on the tested cell lines, and these compound were 3,3ï‚¢,4-tri-O-methylellagic acid (1), 3,3ï‚¢-di-O-methylellagic acid (2), 3-O-methylellagic acid (3) and 3,3ï‚¢-di-O-methylellagic acid-4ï‚¢-O-β-dxylopyranosid (4). Compound 3 showed better obvious radical scavenging activity than other tested compounds.

 

Key words: Euphorbia hylonorma Hand-Mazz, antioxidant activity, cytotoxic activity, chemical constituents.

Abbreviation

DPPH ,1, 1-Diphenyl-2-pycrylhydrazyl; MTT, thiazolyl tetrazolium; DMSO,dimethyl sulfoxide; EDTA, ethylenediaminetetraacetic acid; IC50, inhibition concentration;FBS, fetal bovine serum; PBS, phosphate buffer saline.

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