Abstract

The in vitro and in vivo anti-hypoxia activity of shenjiangsuoyangyiqi drug (SJSY, Chinese medicinal plant mixture) was investigated. The compounds ginsenoside rg1, icariin and peoniflorin were detected in mixture by HPLC-DAD. In vitro anti-hypoxia activity of the mixture was evaluated for MTT assay against two cell lines (Vero and BHK-21). Compared to the control group (Vero: 48.72% and BHK-21: 35.41%), the survival rates of both cells were increased to 87.33% (Vero) and 83.03% (BHK-21) in high-does group. Arresting of the G1 phase of the cell-cycle under hypoxic condition was inhibited by mixture which was analyzed by FCM. In vivo anti-hypoxia activity was studied in mice model.

Key words: Mixture, anti-frigid hypoxia activity, in vitro, in vivo.

Abbreviation

JSYS, Shenjiangsuoyangyiqi; MTT, 3-(4,5-Dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide; FCM, flow cytometry; DAPI, 4', 6-diamidino-2-phenylindole.