In vitro callus induction and plantlet regeneration of Indian red wood, Soymida febrifuga A. Juss (Roxb.)

Kishore K. Chiruvella*; Arifullah Mohammed; Rama Gopal Ghanta; Kishore K. Chiruvella*; Arifullah Mohammed; Kishore K. Chiruvella*; Arifullah Mohammed; Rama Gopal Ghanta

doi:10.5897/JMPR2014.5440

Journal of
Medicinal Plants Research

Abbreviation: J. Med. Plants Res.
Language: English
ISSN: 1996-0875
DOI: 10.5897/JMPR
Start Year: 2007
Published Articles: 3835

Full Length Research Paper

In vitro callus induction and plantlet regeneration of Indian red wood, Soymida febrifuga A. Juss (Roxb.)

Kishore K. Chiruvella*

Kishore K. Chiruvella*
Department of Molecular Biosciences, Stockholm University, Sweden., Division of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
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Arifullah Mohammed

Arifullah Mohammed
Division of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pradesh, India., Faculty of Agrobased Industry, Universiti Malaysia Kelantan, Jeli Campus, Locked bag-100, 17600, Jeli, Kelantan, Malaysia.
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Rama Gopal Ghanta

Rama Gopal Ghanta
Division of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
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Kishore K. Chiruvella*

Kishore K. Chiruvella*
Department of Molecular Biosciences, Stockholm University, Sweden., Division of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
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Arifullah Mohammed

Arifullah Mohammed
Division of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pradesh, India., Faculty of Agrobased Industry, Universiti Malaysia Kelantan, Jeli Campus, Locked bag-100, 17600, Jeli, Kelantan, Malaysia.
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Kishore K. Chiruvella*

Kishore K. Chiruvella*
Department of Molecular Biosciences, Stockholm University, Sweden., Division of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
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Arifullah Mohammed

Arifullah Mohammed
Division of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pradesh, India., Faculty of Agrobased Industry, Universiti Malaysia Kelantan, Jeli Campus, Locked bag-100, 17600, Jeli, Kelantan, Malaysia.
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Rama Gopal Ghanta

Rama Gopal Ghanta
Division of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
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Article Number - A0809CF45914
Vol.8(24), pp. 847-856 , June 2014
https://doi.org/10.5897/JMPR2014.5440

Received: 04 April 2014
Accepted: 19 June 2014
Published: 25 June 2014

Abstract

In vitro seedling explants of Soymida febrifuga were screened for their callus induction and morphogenic potential by culturing on Murashige and Skoog (MS) medium supplemented with naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), indole3acetic acid (IAA) alone or in combination with benzyladenine (BA), kinetin (KN), 2-iP (2-isopentynyl adenine), coconut milk. Multiple shoots were regenerated from cotyledonary node, shoot tip explants derived callus by one stage process where caulogenesis occurred on the primary callus. However cotyledons, leaves and root segments gave rise to adventitious shoots from callus by two stage method of indirect organogenesis. High frequency of shoot differentiation was observed from cotyledon and leaf on MS medium fortified with BA (8.9 to 13.3 μM) in combination with NAA (0.54 μM) compared to other explants. Further, when elongated shoots were subjected to rooting, we observed shoot necrosis consistent to our previous findings. Similarly, we could overcome this problem by supplementing calcium to MS rooting medium. In vitro rooted plantlets were successfully transferred to soil, exhibiting a normal development. Altogether, our protocol provides a rapid technique for mass multiplication as well for ex situ conservation of this endemic medicinal plant.

Key words: Seedling, cotyledon, leaf, shoot necrosis, rooting.

Abbreviation

BA–6, Benzyladenine; CM, coconut milk; CH, casein hydrolysate; 2-iP, 2-isopentynyl adenine; IBA, indole3butyric acid, IAA, indole3acetic acid, KN, kinetin, MS, Murashige and Skoog; CH, casein hydrolysate; PGR, plant growth regulator.