Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3778

Full Length Research Paper

Selective cytotoxicity of Carthamus tinctorius against glucose-deprived HT-29 human colon carcinoma cells

Eun-Soon Son, Jeung-Min Lee, Jeong-Un Choi and Hae-Ryong Park*
Department of Food Science and Biotechnology, Kyungnam University, 449, Wolyoung-dong, Masanhappo-gu, Changwon 631-701, Korea.
Email: [email protected]

  •  Accepted: 11 August 2011
  •  Published: 23 September 2011

Abstract

Glucose deprivation, a feature of poorly vascularized solid tumors, activates the unfolded protein response (UPR) which is a stress-signaling pathway in tumor cells that is associated with the molecular chaperone, glucose-regulated protein 78 (GRP78). Induction of GRP78 protects cells against programmed cell death. Methanolic extract of Carthamus tinctorius(CTE) induced selective cytotoxicity in glucose-deprived HT-29 human colon carcinoma cells; this effect was not seen under normal growth conditions. CTE also suppressed the accumulation of the GRP78 protein and was highly toxic in HT-29 cells, with IC50 values for colony formation of < 50 mg/ml under 2-deoxyglucose (2DG) supplemented and glucose-deprived conditions. Interestingly, apoptotic activity of CTE was also detected by Hoechst staining and flow cytometric analysis. Therefore, these results suggest that CTE prevent the up-regulation of GRP78 and exhibit selective cytotoxicity in glucose-deprived HT-29 cells.

 

Key words: Glucose deprivation, unfolded protein response, (glucose-regulated protein 78) GRP78, Carthamus tinctorius, HT-29 cells.