Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3785

Full Length Research Paper

Optimization of ultrasonic-assisted extraction of phillyrin from Forsythia suspensa using response surface methodology

Zunlai Sheng1, Jichang Li1 and Yanbing Li2*
1College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P. R. China. 2Pharmaceutical College, Heilongjiang University of Chinese Medicine, Harbin 150040, P. R. China.
Email: [email protected]

  •  Accepted: 31 October 2011
  •  Published: 09 March 2012


Phillyrin is an abundant naturally occurring lignan, which makes it a potentially important raw material for a precursor of biologically active compounds. The objective of the current study was to optimize the extraction conditions for phillyrin from Forsythia suspensa. The optimum conditions were evaluated with preliminary experiments and optimized using response surface methodology. Statistical analysis of results showed that the linear and quadric terms of these four variables had significant effects, and evident interaction existing between extraction time and extraction temperature was also found to contribute to the response at a significant level. The extraction conditions were further investigated with Box-Behnken design. The fitted second-order model revealed that the optimal conditions consisted of methanol concentration of 21.17%, extraction time 85.43 min, extraction temperature 66.81°C and ratio of liquid to material 11.2. Under the optimized condition, the experimental value of 0.801±0.012 mg/g was well in close agreement with the value predicted by the model. Moreover, the yield of phillyrin in the condition was higher than that of the non-optimized condition. These results help in designing the process of optimal phillyrin from F. suspensa.


Key words: Forsythia suspensa, phillyrin, optimum conditions, response surface methodology; box-behnken design.