Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3702

Full Length Research Paper

Evaluation of membrane stabilizing, anthelmintic, antioxidant activity with phytochemical screening of methanolic extract of Neolamarckia cadamba fruits

Tairin Islam
  • Tairin Islam
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali - 3814, Bangladesh, India.
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Abhijit Das
  • Abhijit Das
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali - 3814, Bangladesh, India.
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Kumar Bishawjit Shill
  • Kumar Bishawjit Shill
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali - 3814, Bangladesh, India.
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Palash Karmakar*
  • Palash Karmakar*
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali - 3814, Bangladesh, India.
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Shafiul Islam
  • Shafiul Islam
  • Department of Applied Chemistry and Chemical Engineering, Noakhali Science and Technology University, Sonapur, Noakhali - 3814, Bangladesh, India.
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Mohammad Mafruhi Sattar
  • Mohammad Mafruhi Sattar
  • Department of Pharmacy, Jahangirnagar University, Savar, Dhaka - 1342, Bangladesh, India.
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  •  Received: 27 December 2014
  •  Accepted: 02 February 2015
  •  Published: 03 February 2015

Abstract

The current study was directed on methanolic extract of Neolamarckia cadamba fruits, belonging to the family Rubiaceae, to reveal the possible phytochemicals existence and also to evaluate the membrane stabilizing, anthelmintic, antioxidant properties. To estimate the membrane stabilizing activity, both heat and hypotonic solution induced haemolysis techniques were used. The anthelmintic test was conducted on earthworm Pheritima phosthuma using five different concentrations (10, 20, 40, 60, 80 mg/ml) of the extract and albendazole as standard drug (concentration 10 mg/ml).  To investigate antioxidant property, two potential tests namely total phenolic content determination and the 2,2-diphenylpicrylhydrazyl (DPPH) free radical scavenging assay were conducted. Phytochemical screening was carried out using different chemical group tests. The extract revealed good membrane stabilizing activity inhibiting both hypotonic solution and heat induced haemolysis in comparison to inhibition by standard acetyl salicylic acid. The methanolic extract showed potent anthelmintic activity at the highest concentration as it required less time for paralysis and death compared to the standard drug albendazole. The fruit extract showed potential antioxidant property. The analysis of phytochemicals reveals the presence of carbohydrate, phenol, phytosterol, protein and amino acid, terpene and glycoside. The results of the study showed that the plant extract has potential membrane stabilizing, antihelmintic, antioxidant activities along with the presence of significant phytochemicals.

 

Key words: Neolamarckia cadamba, membrane stabilizing activity, anthelmintic activity, antioxidant, phytochemicals.