Abstract

Quercetin 3-O-β-(2"-galloyl)-rhamnopyranoside (QGR) is a polyphenolic compound originally isolated from Persicaria lapathifolia S. F. Gray (Polygonacease). In the present study, we examined the erythropoietic potential of QGR in mouse bone marrow mononuclear cells (BMNCs). BMNCs including hematopoietic stem cells (HSCs), early progenitor cells (EPCs), stromal cells and other precursor cells were isolated from femurs and tibias of 8 to 12-week-old male C57BL/6 mice. QGR significantly increased the cell viability of BMNCs even in hypoxic condition, and mRNA expressions of stem cell factor, granulocyte macrophage-colony stimulating factor and erythropoietin from bone marrow stromal cells. In a colonogenic assay, formation of burst-forming unit-erythroid colony was enhanced by QGR treatment. Immunocytochemistry and flow cytometry against TER-119, a surface marker of erythroid precursor cells revealed that QGR enhanced the number of positive cells. These results suggest that QGR has erythropoietic effect by activating the differentiation of HSCs or EPCs in early stages of hematopoiesis.

Key words: Quercetin 3-O-β-(2"-galloyl)-rhamnopyranoside, bone marrow mononuclear cells, bone marrow stromal cells, burst-forming unit-erythroid, erythropoiesis.