Abstract

The aim of this study was to prepare the formulation of herbal extract mixture in ratio - Ginkgo:Leonuri:Crataegi extracts (1:5:6) with excellent technological properties and identify the phenolic compounds, flavones and flavonols. The flowability study showed that excipients (Prosolv HD90, mannitol+colloidal silicon dioxide and microcrystalline cellulose+colloidal silicon dioxide) reduced powder dustiness and improved powder flow rate index, yet the evaluation of bulk density, tap density, Carr’s index, Hausner ratio, and angle of repose values showed that the herbal powder mixture did not demonstrate excellent powder flow rate index. Wet granulation helped to improve the herbal powder flow rate index by 1.7 - 2.0 fold, control powder density, and control the tendency of powders to segregate. The results showed that different binder solutions had different effect on powder flow rate index. Granulation of the maidenhair tree, hawthorn, and motherwort extract powder using a natural and related binder solution - motherwort extract ethanolic solution - resulted in the lowest particle size variation. In addition, natural binder solutions had different effects on powder flow rate index and particle size, and provided the powder with better technological properties than chemical binder solutions such as ethanol or ethanolic povidone solution did. The amount of phenolic compounds, flavones and flavonols identified in the mixture of three herbal extracts determines its antioxidant activity.

Key words: Phenolic compounds, flowability, Ginkgo biloba, Leonurus cardiaca,Crataegus monogyna.