The choice of substances that are not harmful to man and the environment is a priority in the production of sanitizing products. The aim of this study was to evaluate the disinfectant action and biofilm biomass reduction of the ethanolic extract of Genipa americana fruit. The extract disinfectant efficacy was assessed by modified Kelsey-Sykes, solid surface, and use-dilution methods. Also, the ability of the extract to disrupt biofilm mass was evaluated. Standard disinfectants and Gentamicin were used as control. Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, MRSA and MLSB Staphylococcus hominis and Staphylococcus haemolyticus and Staphylococcus species were used as infective and biofilm-forming microorganisms. G. americana fruit extract was not as effective as C12-C14 and BCB, however, it was more effective over Staphylococcus bacteria than 70% alcohol on stainless steel surface for a shorter exposure time. The extract did not show the ability to remove adhered P. aeruginosa nor to disrupt the mature biofilm. The ethanolic extract of genipap did not show optimal disinfectant action in the simulated critical environment; however, considering the reported antimicrobial action of this extract, more studies should be considered to find the best formula to improve this extract's disinfectant efficacy.
Key words: Genipa americana extract, disinfectant, biofilm, microorganisms.
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